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MABS64

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Anti-phospho-p38 (Thr180/Tyr182) Antibody, clone 6E5.2

clone 6E5.2, from mouse

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Synonym(s):
mitogen-activated protein kinase 14, cytokine suppressive anti-inflammatory drug binding protein, stress-activated protein kinase 2A, Cytokine suppressive anti-inflammatory drug-binding protein, CSAID-binding protein, MAP kinase MXI2, MAX-interacting pro
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

6E5.2, monoclonal

species reactivity

human, mouse

technique(s)

immunocytochemistry: suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

phosphorylation (pThr180/pTyr182)

Gene Information

human ... MAPK14(1432)

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SAB4500493SAB4500492SAB4500490
antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

biological source

mouse

biological source

rabbit

biological source

rabbit

biological source

rabbit

species reactivity

human, mouse

species reactivity

human, rat, mouse

species reactivity

human, rat, mouse

species reactivity

mouse, human, rat

clone

6E5.2, monoclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

technique(s)

immunocytochemistry: suitable, western blot: suitable

technique(s)

ELISA: 1:10000, western blot: 1:500-1:1000

technique(s)

ELISA: 1:20000, western blot: 1:500-1:1000

technique(s)

ELISA: 1:1000, immunofluorescence: 1:100-1:500, immunohistochemistry: 1:50-1:100, western blot: 1:500-1:1000

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General description

The p38 family refers to the SAPK2/3 Kinases (Stess-Activated Protein Kinases), a subfamily of JNK/SAPK family. p38/SAPK Kinases are also activated by stresses, most notably inflammatory cytokines, irradiation, and certain toxins such as anisomycin and arsenite. SAPKs have been implicated in many pathological conditions, including inflammation, cancer and neurodegenerative diseases. As with the other MAPK family members (ERK, JNK, p38MAPK, and ERK5), p38 is activated through phosphorylation on both threonine and tyrosine residues at the Thr-X-Tyr dual phosphorylation motif, where X is Gly for p38. Once phosphorylated and active, it then goes on to phosphorylate downstream targets in both the cytoplasm and the nucleus. Some of their main targets of p38 are the kinase MAPKAPK2 and the transcription factor ATF2 that are activated via their phosphorylation.

Specificity

This antibody recognizes p38 when phosphorylated at Thr180 and Tyr182.

Immunogen

Epitope: Phosphorylated Thr180 and Tyr182
Linear peptide corresponding to human p38 phosphorylated at Thr180 and Tyr182.

Application

Detect phospho-p38 (Thr180/Tyr182) using this Anti-phospho-p38 (Thr180/Tyr182) Antibody, clone 6E5.2 validated for use in WB & IC.
Immunocytochemistry Analysis: 1:500 dilution from a previous lot detected p38 in Anisomycin treated NIH/3T3 cells.
Research Category
Signaling
Research Sub Category
MAP Kinases

Quality

Evaluated by Western Blot in Anisomycin treated and untreated HeLa cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected p38 on 10 µg of Anisomycin treated and untreated HeLa cell lysate.

Target description

41 kDa observed

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgGκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Anisomycin treated and untreated HeLa cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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