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MABT395

Sigma-Aldrich

Anti-Muc4 Antibody, clone 8G7

clone 8G7, from mouse

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Synonym(s):
Mucin-4 beta chain
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

8G7, monoclonal

species reactivity

human

technique(s)

immunohistochemistry: suitable
western blot: suitable

isotype

IgG1κ

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... MUC4(4585)

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This Item
WH0004585M7AV41446406M-1
antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

IgG fraction of antiserum

antibody form

culture supernatant

clone

8G7, monoclonal

clone

5B12, monoclonal

clone

polyclonal

clone

8G7, monoclonal

species reactivity

human

species reactivity

human

species reactivity

dog, human

species reactivity

human

technique(s)

immunohistochemistry: suitable, western blot: suitable

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, indirect ELISA: suitable, western blot: 1-5 μg/mL

technique(s)

immunohistochemistry: suitable, western blot: suitable

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:10-1:50

isotype

IgG1κ

isotype

IgG2aκ

isotype

-

isotype

IgG1κ

General description

Mucin-4 (MUC-4 or Muc4) is also called Ascites sialoglycoprotein (ASGP), Pancreatic adenocarcinoma mucin, Testis mucin, and Tracheobronchial mucin. Muc4 is proteolytically cleaved into two chains: mucin-4 alpha chain and mucin-4 beta chain. Muc4 has anti-adhesive properties and is involved in tumor progression, cell proliferation and differentiation of epithelial cells. Muc4 is expressed in the thymus, thyroid, lung, trachea, esophagus, stomach, small intestine, colon, testis, prostate, ovary, uterus, placenta, mammary and salivary glands as well as lung cancers, squamous cell carcinomas of the upper aerodigestive tract, mammary carcinomas, biliary tract, colon, cervix cancers, pancreatic tumors, and pancreatic tumor cell lines. The expression of Muc4 has become a very useful indicator of poor prognosis in patients with invasive ductal carcinoma and intrahepatic cholangiocarcinoma, mass forming type (IDC,ICC-MF), where patients with high Muc4 expression had lower survival rates than those with low Muc4 expression.

Immunogen

KLH-conjugated linear peptide corresponding to the beta chain tandem repeat region of human Muc4.

Application

Anti-Muc4 Antibody, clone 8G7 is a highly specific mouse monoclonal antibody, that targets Mucin & has been tested in western blotting & IHC.
Immunohistochemistry Analysis: A 1:2,000 dilution from a representative lot detected Muc4 in human ilium tissues.

Western Blotting Analysis: A representative lot from an independent laboratory detected Muc4 in maligant melanoma and cutaneous squamous cell carcinoma tissue lysates (Moniaux, N., et al. (2004). J Histochem Cytochem. 52(2):253-261.).

Immunohistochemistry Analysis: A representative lot from an independent laboratory detected Muc4 in lung adenocarcinoma and pancreatic adenocarcinoma tissues (Moniaux, N., et al. (2004). J Histochem Cytochem. 52(2):253-261.).

Immunohistochemistry Analysis: A representative lot from independent laboratories detected Muc4 in 330 tissue spots representing normal skin, and benign and malignant cutaneous diseases (Singh, A. P., et al. (2006). Prostate. 66(4):421-429.; Chakraborty, S., et al. (2010). J Clin Pathol. 63(7):579-584.).
Research Category
Cell Structure
Research Sub Category
ECM Proteins

Quality

Evaluated by Western Blotting in CD18/HPAF cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected Muc4 in 10 µg of CD18/HPAF cell lysate.

Target description

~240 kDa observed. Uniprot describes a molecular weight at 430 kDa; however, this protein may be observed at ~240 kDa due to alternative spicing and post-translational modification (Moniaux, N., et al. (2004). J Histochem Cytochem. 52(2):253-261.).

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl without preservatives.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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