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MABT522

Sigma-Aldrich

Anti-alpha-Tubulin Antibody, clone RM113

clone RM113, from rabbit

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Synonym(s):
Tubulin alpha-1B chain, Alpha-tubulin ubiquitous, Tubulin K-alpha-1, Tubulin alpha-ubiquitous chain, alpha-Tubulin
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

RM113, monoclonal

species reactivity

human

technique(s)

immunocytochemistry: suitable
western blot: suitable

isotype

IgG

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... TUBA1A(7846)

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ABT171MABT205SAB5600206
clone

RM113, monoclonal

clone

polyclonal

clone

DM1A, monoclonal

clone

RM113, monoclonal, recombinant monoclonal

antibody form

purified antibody

antibody form

serum

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

species reactivity

human

species reactivity

monkey, mouse

species reactivity

mouse, human

species reactivity

all

Gene Information

human ... TUBA1A(7846)

Gene Information

human ... TUBA1A(7846)

Gene Information

human ... TUBA1A(7846)

Gene Information

human ... TUBA1A(7846)

UniProt accession no.

P68363

UniProt accession no.

Q71U36

UniProt accession no.

P02552

UniProt accession no.

P68363

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General description

Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Tubulin itself is a globular protein which consists of two polypeptides (alpha and beta tubulin). Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22 nm diameter. Tyrosine ligase adds a C-terminal tyrosin to monomeric alpha tubulin. Assembled microtubules can be detyrosinated by a cytoskeleton associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation which is characteristic for microtubules in neuronal cells and the mitotic spindle.

Immunogen

A peptide corresponding to the C-terminus of α-Tubulin.

Application

Immunocytochemistry Analysis (ICC): A 1:200 dilution from a representative lot detected alpha-Tubulin in A431 cells.

This Anti-alpha-Tubulin Antibody, clone RM113 is validated for use in Western Blotting, Immunocytochemistry for the detection of alpha-Tubulin.

Quality

Evaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected alpha-Tubulin in A431 cell lysate.

Target description

~53 kDa observed. Uncharacterized bands may be observed in some lysate(s).

Physical form

Format: Purified

Other Notes

Concentration: Please refer to lot specific datasheet.

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Wei Yu et al.
Frontiers in cell and developmental biology, 10, 918235-918235 (2023-01-03)
SATB homeobox proteins are important regulators of developmental gene expression. Among the stem cell lineages that emerge during early embryonic development, trophoblast stem (TS) cells exhibit robust SATB expression. Both SATB1 and SATB2 act to maintain the trophoblast stem-state. However

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