MABT96

Anti-Anillin Antibody, clone 5f3.1

clone 5F3.1, from mouse

• Synonym(s): Actin-binding protein anillin
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 5F3.1, monoclonal
• species reactivity: human
• technique(s): immunocytochemistry: suitable; western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_061155
• UniProt accession no.: Q9NQW6
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... ANLN(54443)

Description

General description

Anillin is a highly conserved multidomain protein that crosslinks multiple structural and regulatory elements including, but not limited to, F-actin, active myosin II, septins, and importin. It positively regulates normal cytokinesis; however, its molecular function within this context remains unclear. Failed cytokinesis results in binucleation, which can lead to genomic instability. In human cultured cells, anillin stabilizes TCA-fixable species of RhoA at the equatorial cortex, but its exact mode of action and subsequent physiological effects have yet to be determined. In some cancers, high levels of septins are shown to out-compete importin binding, thereby promoting the retention of Anillin in the cytoplasm.

Immunogen

GST-tagged recombinant protein corresponding to human Anillin.

Application

Anti-Anillin, clone 5F3.1 is an antibody targeting the Anillin protein, validated for use in WB & ICC.

Immunocytochemistry Analysis: 1 µg/mL of this antibody detected Anillin in the contractile rings of dividing HeLa cells.

Research Category
Cell Structure

Research Sub Category
Cytoskeletal Signaling

Quality

Evaluated by Western Blot in mimosine and nocodozole treated and untreated HeLa cell lysate.
Western Blot Analysis: 1.0 µg/mL of this antibody detected Anillin in 10 µg of mimosine and nocodozole treated and untreated HeLa cell lysate.

Evaluated by Immunohistochemistry (Paraffin) in human testis tissue sections.
Immunohistochemistry (Paraffin) Analysis (IHC(P)): A 1:100 dilution of this antibody detected Anillin in human testis tissue sections.

Target description

~160 kDa observed. The calculated molecular weight of this protein is 124 kDa, but can be observed at ~160 kDa due to high glycosylation. Uniprot describes an isoform produced by alternative splicing at ~120 kDa

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Mimosine and nocodozole treated and untreated HeLa cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable