S4117
Formamide, Deionized
Synonym(s):
DI formamide, formamide for hybridization
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About This Item
UNSPSC Code:
41116012
eCl@ss:
32160405
NACRES:
NA.21
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packaging
pkg of 500 mL
Quality Level
manufacturer/tradename
Chemicon®
technique(s)
hybridization: suitable
shipped in
wet ice
General description
This product is intended for use in hybridization applications for in situ, Southern, Northern, dot and slot blot analyses, and colony, plaque, and YAC screening. Formamide is used primarily as a component in probe hybridization solutions to promote probe hybridization specificity. It denatures nucleic acids by disrupting hydrogen bonds.
Application
Dilute formamide according to specific application requirements. Formamide concentrations of 45-50% are typically used in Southern and Northern analyses and a 50% or greater concentration for in situ hybridization techniques. In DNA to DNA hybridization, each 1% increase in formamide concentration lowers the Tm by 0.7%.
Research Category
All
All
Quality
Each lot of material is pre-filtered through a mixed-ion exchange resin to eliminate products of decomposition and residual metal cations. This filtering process guarantees that each lot has a final purity of greater than 99.5% and an adsorbance value of less than 0.02 adsorbance units at 280nm. Each lot then undergoes rigid quality control testing to insure that assay specifications are met prior to release.
Physical form
This preperation of formamide is deionized, triple distilled and packaged under nitrogen and is free of detectable DNAse, RNAse, and protease activity.
Storage and Stability
Store at -20°C. Keep cap tightly closed after opening or aliquot and store at -15°C to -25°C.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
signalword
Danger
hcodes
Hazard Classifications
Carc. 2 - Repr. 1B - STOT RE 2 Oral
target_organs
Blood
Storage Class
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
wgk_germany
WGK 1
flash_point_f
305.6 °F
flash_point_c
152 °C
Certificates of Analysis (COA)
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