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47671

Sigma-Aldrich

Formamide

BioUltra, for molecular biology, ≥99.5% (T)

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Synonym(s):
Amide C1, Formic amide
Linear Formula:
HCONH2
CAS Number:
Molecular Weight:
45.04
Beilstein:
505995
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.25

grade

for molecular biology

vapor density

1.55 (vs air)

vapor pressure

0.08 mmHg ( 20 °C)
30 mmHg ( 129 °C)

product line

BioUltra

Assay

≥99.5% (T)

form

liquid

autoignition temp.

932 °F

technique(s)

hybridization: suitable

impurities

DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected

ign. residue

≤0.1%

refractive index

n20/D 1.447 (lit.)
n20/D 1.447

pH

7-9 (25 °C, 1 M in H2O)

bp

210 °C (lit.)

mp

2-3 °C (lit.)

solubility

H2O: 10 M at 20 °C, clear, colorless

density

1.134 g/mL at 25 °C (lit.)

anion traces

chloride (Cl-): ≤1 mg/kg
sulfate (SO42-): ≤10 mg/kg

cation traces

Ag: ≤5 mg/kg
Al: ≤1 mg/kg
As: ≤0.1 mg/kg
Ba: ≤1 mg/kg
Bi: ≤1 mg/kg
Ca: ≤5 mg/kg
Cd: ≤1 mg/kg
Co: ≤1 mg/kg
Cr: ≤1 mg/kg
Cu: ≤1 mg/kg
Fe: ≤1 mg/kg
K: ≤20 mg/kg
Li: ≤1 mg/kg
Mg: ≤1 mg/kg
Mn: ≤1 mg/kg
Mo: ≤1 mg/kg
NH4+: ≤1000 mg/kg
Na: ≤20 mg/kg
Ni: ≤1 mg/kg
Pb: ≤1 mg/kg
Sr: ≤1 mg/kg
Tl: ≤5 mg/kg
Zn: ≤1 mg/kg

SMILES string

NC=O

λ

10 M in H2O

UV absorption

λ: 260 nm Amax: ≤0.4
λ: 280 nm Amax: ≤0.1

InChI

1S/CH3NO/c2-1-3/h1H,(H2,2,3)

InChI key

ZHNUHDYFZUAESO-UHFFFAOYSA-N

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This Item
F9037344206-M1.04008
Formamide BioUltra, for molecular biology, ≥99.5% (T)

Sigma-Aldrich

47671

Formamide

Premium Grade
Formamide ≥99.5% (GC), BioReagent, for molecular biology

Sigma-Aldrich

F9037

Formamide

-
Formamide EMPLURA®

Supelco

1.04008

Formamide

-
vapor density

1.55 (vs air)

vapor density

1.55 (vs air)

vapor density

1.55 (vs air)

vapor density

1.55 (vs air)

vapor pressure

0.08 mmHg ( 20 °C), 30 mmHg ( 129 °C)

vapor pressure

0.08 mmHg ( 20 °C), 30 mmHg ( 129 °C)

vapor pressure

0.08 mmHg ( 20 °C), 30 mmHg ( 129 °C)

vapor pressure

0.08 mmHg ( 20 °C), 30 mmHg ( 129 °C)

assay

≥99.5% (T)

assay

≥99.5% (GC)

assay

-

assay

≥99% (N)

form

liquid

form

liquid

form

liquid

form

liquid

autoignition temp.

932 °F

autoignition temp.

932 °F

autoignition temp.

932 °F

autoignition temp.

932 °F

General description

Formamide, a naturally occurring amide, contains carbon, hydrogen, oxygen, and nitrogen in its structure.

Application

Formamide has been used:
  • as a component in the hybridization buffer for in situ hybridization as a component of solution X to wash the slides to remove the coverslips
  • in the preparation of denaturation solution for spectral karyotyping (SKY) analysis
  • in the preparation of 1% formaldehyde solution for the pretreatment of slides for comparative genomic hybridization (CGH)
  • to prepare pre-hybridization buffer I, II, III, IV, and hybridization buffer I, II, and III for RNA fluorescence in situ hybridization (RNA-FISH)
  • as an ionizing solvent in the production of formic esters and vitamin, a softener for paper, in animal glues, and water-soluble gums, in molecular biology as a denaturing agent

Formamide destabilizes nucleic acid duplexes and may be used, typically, at a concentration of 50%, in hybridization protocols requiring lower hybridization temperatures.

Biochem/physiol Actions

Formamide serves as a good solvent for polar organic and inorganic compounds with potential catalytic activity in prebiotic processes due to its high dielectric constant.

Other Notes

For the preparation of formamide denaturing polyacrylamide gels; In situ hybridization on human chromosomes

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Hazard Classifications

Carc. 2 - Repr. 1B - STOT RE 2 Oral

Target Organs

Blood

Storage Class Code

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 1

Flash Point(F)

305.6 °F

Flash Point(C)

152 °C

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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R.C. Ogden et al.
Methods in Enzymology, 152, 67-67 (1987)
G. Wilkinson, ed.
In Situ Hybridization: A practical approach, 147-147 (1992)
Min Chen et al.
Prenatal diagnosis, 35(6), 534-543 (2015-02-03)
To develop a single-tube multi-marker assay for improved preimplantation genetic diagnosis (PGD) of deletional and/or non-deletional Hb Bart's hydrops fetalis syndrome, providing haplotype confirmation of deletional status, and maximization of linkage informativity. We performed in silico mining to identify novel
A Touati et al.
Journal of clinical microbiology, 53(10), 3182-3194 (2015-07-24)
Molecular typing of Mycoplasma pneumoniae is an important tool for identifying grouped cases and investigating outbreaks. In the present study, we developed a new genotyping method based on single nucleotide polymorphisms (SNPs) selected from the whole-genome sequencing of eight M.
Jing Wang et al.
The journal of physical chemistry. B, 117(8), 2314-2320 (2013-01-26)
A step-by-step mechanistic pathway following the transformation of formamide to purine through a five-membered ring intermediate has been explored by density functional theory computations. The highlight of the mechanistic route detailed here is that the proposed pathway represents the simplest

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