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F7503

Sigma-Aldrich

Formamide

ReagentPlus®, ≥99.0% (GC)

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Synonym(s):
Amide C1, Formic amide
Linear Formula:
HCONH2
CAS Number:
Molecular Weight:
45.04
Beilstein:
505995
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.21

vapor density

1.55 (vs air)

vapor pressure

0.08 mmHg ( 20 °C)
30 mmHg ( 129 °C)

product line

ReagentPlus®

Assay

≥99.0% (GC)

form

liquid

autoignition temp.

932 °F

expl. lim.

2.7-19 %

technique(s)

hybridization: suitable

refractive index

n20/D 1.447 (lit.)

pH

4-10 (20 °C, 200 g/L)

bp

210 °C (lit.)

mp

2-3 °C (lit.)

density

1.134 g/mL at 25 °C (lit.)

SMILES string

NC=O

InChI

1S/CH3NO/c2-1-3/h1H,(H2,2,3)

InChI key

ZHNUHDYFZUAESO-UHFFFAOYSA-N

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1 of 4

This Item
47671221198F9037
Formamide ReagentPlus®, ≥99.0% (GC)

Sigma-Aldrich

F7503

Formamide

-
Formamide BioUltra, for molecular biology, ≥99.5% (T)

Sigma-Aldrich

47671

Formamide

Premium Grade
Formamide ACS reagent, ≥99.5%

Sigma-Aldrich

221198

Formamide

-
Formamide ≥99.5% (GC), BioReagent, for molecular biology

Sigma-Aldrich

F9037

Formamide

-
vapor pressure

0.08 mmHg ( 20 °C), 30 mmHg ( 129 °C)

vapor pressure

0.08 mmHg ( 20 °C), 30 mmHg ( 129 °C)

vapor pressure

0.08 mmHg ( 20 °C), 30 mmHg ( 129 °C)

vapor pressure

0.08 mmHg ( 20 °C), 30 mmHg ( 129 °C)

assay

≥99.0% (GC)

assay

≥99.5% (T)

assay

≥99.5%

assay

≥99.5% (GC)

form

liquid

form

liquid

form

liquid

form

liquid

autoignition temp.

932 °F

autoignition temp.

932 °F

autoignition temp.

932 °F

autoignition temp.

932 °F

expl. lim.

2.7-19 %

expl. lim.

-

expl. lim.

-

expl. lim.

-

General description

Formamide is a polar solvent mainly used for resins and plasticizers. It can be prepared by direct synthesis from carbon monoxide and ammonia.

Application

Formamide can be used as a solvent:
  • To synthesize tetrakis(trialkylsilyl) diphosphate by reacting disodium dihydrogen diphosphate with trialkyl chlorosilane.
  • In Cu/C catalyzed cycloaddition between azides and terminal alkynes to synthesize triazoles.
Formamide destabilizes nucleic acid duplexes and may be used, typically, at a concentration of 50%, in hybridization protocols requiring lower hybridization temperatures.

Legal Information

ReagentPlus is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Hazard Classifications

Carc. 2 - Repr. 1B - STOT RE 2 Oral

Target Organs

Blood

Storage Class Code

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 1

Flash Point(F)

305.6 °F

Flash Point(C)

152 °C


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The crystal structure of formamide.
Ladell J and Post B.
Acta Crystallographica, 7(8-9), 559-564 (1954)
The formamide method for the extraction of polysaccharides from haemolytic streptococci.
Fuller AT.
British Journal of Experimental Pathology, 19(2), 130-130 (1938)
Microwave spectrum, structure, dipole moment, and quadrupole coupling constants of formamide.
Kurland RJ and Wilson Jr EB.
J. Chem. Phys. , 27(2), 585-590 (1957)
The dynamics of liquid formamide, N-methylformamide, N,N-dimethylformamide, and N,N-dimethylacetamide. A dielectric relaxation study.
Barthel J, et al.
Journal of Molecular Liquids, 98, 51-69 (2002)
Formamide can dramatically improve the specificity of PCR.
G Sarkar et al.
Nucleic acids research, 18(24), 7465-7465 (1990-12-25)

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