MilliporeSigma
All Photos(3)

SCC064

Sigma-Aldrich

LX-2 Human Hepatic Stellate Cell Line

The LX-2 human hepatic stellate cell line has been extensively characterized and retain key features of hepatic stellate cytokine signaling, neuronal gene expression, retinoid metabolism, and fibrogenesis, making them a highly suitable model of human hepatic fibrosis.

Sign Into View Organizational & Contract Pricing

Select a Size

Synonym(s):
HSC, Perisinusoidal cells, Ito cells, Hepatic lipocytes, Hepatic pericytes
eCl@ss:
32011203
NACRES:
NA.75

biological source

human

Quality Level

technique(s)

cell culture | stem cell: suitable

shipped in

liquid nitrogen

Related Categories

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
SCC043SCC109SCC049
technique(s)

cell culture | stem cell: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | stem cell: suitable

shipped in

liquid nitrogen

shipped in

ambient

shipped in

dry ice

shipped in

liquid nitrogen

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

General description

Hepatic stellate cells are a major cell type responsible for liver fibrosis following their activation into fibrogenic myofibroblast-like cells in diseases such as chronic alcoholism, hepatitis B and C, fatty liver disease, obesity and diabetes. There is an increasing need for renewable cell culture models that faithfully recapitulate their in vivo phenotype, particularly for human studies.

LX-2 was generated by immmortalization of primary human hepatic stellate cells with the SV40 large T antigen followed by selective culture of early passaged cells in low serum media conditions.

Immortalized LX-2 was established by Xu et al to overcome issues of culture variability and to provide a stable and unlimited source of human hepatic stellate cells that are homogeneous. These cell lines have been extensively characterized and retain key features of cytokine signaling, neuronal gene expression, retinoid metabolism, and fibrogenesis, making them highly suitable for culture based studies of human hepatic fibrosis.

Application

Research Category
Infectious Diseases

Inflammation & Immunology

Stem Cell Research

Components

1) ≥1X106 viable LX-2 Human Hepatic Stellate Cells: (Catalog No. SCC064). Store in liquid nitrogen.

Quality

• Each vial contains ≥ 1X106 viable cells.
• Cells are tested by PCR and are negative for Hepatitis A, B, C and HIV-1 & 2 viruses.
• Cells are negatrive for mycoplasma contamination.

Storage and Stability

LX-2 cells should be stored in liquid nitrogen. The cells can be passage for at least 10 passages without significantly affecting the cell marker expression and functionality. LX-2 cells have been successfully expanded past passage 50 in culture.

Other Notes

This product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the "Academic Use Agreement" as detailed in the product documentation. For information regarding any other use, please contact licensing@emdmillipore.com.

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Customers Also Viewed

Slide 1 of 2

1 of 2

Genetic characteristics of the human hepatic stellate cell line LX-2.
Weiskirchen, Ralf, et al.
Testing, 8, e75692-e75692 (2013)
Epigenetic regulation of connective tissue growth factor by microRNA-214 delivery in exosomes from mouse or human hepatic stellate cells.
Chen, Li, et al.
Hepatology (2013)
Yong Rao et al.
British journal of pharmacology, 176(16), 2877-2893 (2019-05-22)
Non-alcoholic hepatic fatty liver disease (NAFLD) is a manifestation of the metabolic syndrome in the liver and non-alcoholic steatohepatitis (NASH) represents its advanced stage. R17 derived from bouchardatine, shows benefits in the metabolic syndrome, but has not been tested in
Expression of Septin4 in human hepatic stellate cells LX-2 stimulated by LPS.
Sun, Xiaolei, et al.
Inflammation, 36, 539-548 (2013)
Human hepatic stellate cell lines, LX-1 and LX-2: new tools for analysis of hepatic fibrosis.
Xu, L, et al.
Gut, 54, 142-151 (2005)

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service