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Mesenchymal Stem Cell Expansion Medium (1x)

The Mesenchymal Stem Cell Expansion Medium (1x) has been optimized & validated for Stem cell culture. This medium is available in a 500mL format.

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eCl@ss:
32160801
NACRES:
NA.75

Quality Level

form

liquid

manufacturer/tradename

Chemicon®

technique(s)

cell culture | stem cell: suitable

input

sample type mesenchymal stem cell(s)

shipped in

dry ice

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SCM002SCM003SCM001
technique(s)

cell culture | stem cell: suitable

technique(s)

cell culture | stem cell: suitable

technique(s)

cell culture | stem cell: suitable

technique(s)

cell culture | stem cell: suitable

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

Quality Level

200

Quality Level

300

Quality Level

300

Quality Level

300

manufacturer/tradename

Chemicon®

manufacturer/tradename

Chemicon®

manufacturer/tradename

Chemicon®

manufacturer/tradename

Chemicon®

input

sample type mesenchymal stem cell(s)

input

sample type: mouse embryonic stem cell(s)
sample type induced pluripotent stem cell(s)

input

sample type neural stem cell(s)

input

sample type induced pluripotent stem cell(s)
sample type: mouse embryonic stem cell(s)

Application


Thawing of Cells:

1. Do not thaw the cells until the recommended medium and appropriate plasticware and/or glassware are on hand.
2. Remove the vial of mesenchymal stem cells from liquid nitrogen and incubate in a 37°C water bath. Closely monitor until the cells are completely thawed. Maximum cell viability is dependent on the rapid and complete thawing of frozen cells. IMPORTANT: Do not vortex the cells.
3. As soon as the cells are completely thawed disinfect the outside of the vial with 70% ethanol. Proceed immediately to the next step.
4. In a laminar flow hood, use a 1 or 2 mL pipette to transfer the cells to a sterile 15 mL conical tube. Be careful to not introduce any bubbles during the transfer process.
5. Using a 10 mL pipette, slowly add dropwise 9 mL of Mesenchymal Stem Cell Expansion Medium (pre-warmed at 37°C) to the 15 mL conical tube. IMPORTANT: Do not add the whole volume of medium at once to the cells. This may result in decreased cell viability due to osmotic shock.
6. Gently mix the cell suspension by slow pipeting up and down twice. Be careful to not introduce any bubbles. IMPORTANT: Do not vortex the cells.
7. Centrifuge the tube at 300 x g for 2-3 minutes to pellet the cells.
8. Decant as much of the supernatant as possible. Steps 4-8 are necessary to remove residual cryopreservative (DMSO).
9. Resuspend the cells in a total volume of 10 mL of Mesenchymal Stem Cell Expansion Medium (pre-warmed to 37°C).
10. Plate the cell mixture onto a 10-cm tissue culture plate.
11. Incubate the cells at 37°C in a 5% CO2 humidified incubator.
12. The next day, exchange the medium with fresh Mesenchymal Stem Cell Expansion Medium (pre-warmed to 37°C). Exchange with fresh medium every two to three days thereafter.
13. When the cells are approximately 80% confluent, they can be dissociated with Accutase® and passaged or alternatively frozen for later use.

Subculturing:

1. Carefully remove the medium from the 10-cm tissue culture plate containing the confluent layer of mesenchymal stem cells.
2. Apply 3-5 mL of Accutase and incubate in a 37°C incubator for 3 minutes.
3. Inspect the plate and ensure the complete detachment of cells by gently tapping the side of the plate with the palm of your hand. 4. Apply 5 mL of Mesenchymal Stem Cell Expansion Medium (pre-warmed to 37°C) to the plate.
5. Transfer the dissociated cells to a 15 mL conical tube.
6. Centrifuge the tube at 300 x g for 2-3 minutes to pellet the cells.
7. Discard the supernatant
8. Apply 2 mL of Mesenchymal Stem Cell Expansion Medium to the conical tube and resuspend the cells thoroughly. IMPORTANT: Do not vortex.
9. Count the number of cells using a hemacytometer.
10. Plate the cells to the desired density into the appropriate flasks, plates or wells in Mesenchymal Stem Cell Expansion. We typically plate the cells at ~2 million cells per 10-cm plate or T75 flask.

Quality

Each lot of medium is tested for the optimal cell growth and proliferation of mesenchymal stem cells. Sterility Testing: Negative

Physical form

Mesenchymal Stem Cell Expansion Medium is a proprietary formulation that contains fetal bovine serum.

Storage and Stability

Mesenchymal Stem Cell Expansion Medium should be stored at -20°C until ready to use. Upon thawing, the basal medium should be stored at 2-8°C for up to one month.

Legal Information

Accutase is a registered trademark of Innovative Cell Technologies, Inc.
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Articles

Development of a novel serum-free and xeno-free human mesenchymal stem cell (MSC) osteocyte differentiation media.

PLTMax® Human Platelet Lysate (hPL) is a superior serum-free and xeno-free media supplement alternative to fetal bovine serum (FBS) for human mesenchymal stem cell (MSC) cultures.

Frequently asked questions about mesenchymal stem cells including MSC derivation, expansion, differentiation and allogenic stem cell therapy.

Protocols

Information about mesenchyme, specifically mesenchymal stem cell procotols. Step-by-step cell culture protocols for mesenchymal stem cell (MSC) isolation, expansion and differentiation.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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