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SCM302

Sigma-Aldrich

Definitive Endoderm Induction Medium

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Select a Size

form

liquid

packaging

pkg of 50 mL

technique(s)

cell culture | stem cell: suitable

impurities

<2 EU/mL endotoxin

pH

7.0-7.4

application(s)

cell analysis

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SCM018SCM121SCM304
packaging

pkg of 50 mL

packaging

-

packaging

-

packaging

pkg of 50 mL

technique(s)

cell culture | stem cell: suitable

technique(s)

cell differentiation: suitable

technique(s)

cell culture | stem cell: suitable

technique(s)

cell culture | stem cell: suitable

impurities

<2 EU/mL endotoxin

impurities

-

impurities

-

impurities

-

application(s)

cell analysis

application(s)

-

application(s)

-

application(s)

cell analysis

pH

7.0-7.4

pH

-

pH

-

pH

-

General description

The definitive endoderm generates the epithelial lining of the respiratory and digestive tracts as well as cell of the thyroid, thymus, lungs, liver and pancreas. The definitive endoderm induction medium (SCM302) is a defined, animal component-free, ready-to-use serum-free medium for efficient differentiation of human pluripotent stem cells (ES/iPSCs) to definitive endoderm (DE) cells.

The 3-day differentiation protocol is simple and has been validated on multiple human iPS cell lines. Differentiated cells express high levels (>85%) of endoderm markers including CXCR4, c-Kit, Sox-17 and FOXA2 and can be further differentiated into specialized endodermal cell lineages. Definitive Endoderm Induction Medium can be used to generate 3D organoids such as hiPS derived colon (Cat. No. SCC300) and lung organoids.

Application

Ready-to-use serum-free media for the efficient differentiation of human pluripotent stem cells (ES/iPSCs) to CXCR4+/c-Kit+/Sox-17+/FOXA2+ definitive endoderm (DE) cells.

Research Category:
Stem Cell Research

Quality

Appearance: Clear liquid/no particulates
Sterility Tested: No growth
Endotoxin: <2 EU/mL
pH: 7.0-7.4
Mycoplasma: Negative
Functional Assay: >85% CXCR4+ c-Kit+ double positive after 3 days of induction.

Storage and Stability

Upon receipt, store at -20°C. When ready to use, thaw overnight at 2-8°C. Once thawed, mix thoroughly and aliquot into smaller volumes (i.e. 10 mL), use immediately and store at 2-8°C for up to 1 week. Do not re-freeze. Unused aliquots may be stored at -20°C until the expiry date.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Articles

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Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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