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SCM303

Hindgut Induction Medium

Ready-to-use serum-free media used for the efficient differentiation of iPSC derived definitive endoderm (DE) cells to CDX-2+ hindgut endoderm cells.

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form

liquid

packaging

pkg of 50 mL

technique(s)

cell culture | stem cell: suitable

impurities

<2 EU/mL endotoxin

pH

7.0-7.4

General description

The hindgut is the region of the gut tube lying at the distal third of the transverse colon and gives rise to splenic flexure, descending colon, sigmoid colon and rectum. Additionally, the endodermal layer of the hindgut region forms the epithelial lining of the urinary bladder and urethra. The main function of the hindgut is to absorb most of the water and nutrients remaining from partially digested food. The Hindgut Induction Medium is a defined ready-to-use serum-free media used for the efficient differentiation of definitive endoderm cells to CDX-2 positive hindgut endoderm cells. The 4-day differentiation protocol is simple and has been validated on two human iPS cell lines. Differentiated cells express high levels of the hindgut endoderm marker, CDX-2. CDX-2 hindgut endoderm cells can be further differentiated into specialized lineages such as intestinal colon organoids after embedding in Matrigel and cultured in 3dGRO Human Colon Organoid Expansion Medium (SCM304).

Application

Ready-to-use serum-free media used for the efficient differentiation of iPSC derived definitive endoderm (DE) cells to CDX-2+ hindgut endoderm cells.

Research Category:
  • Stem Cell Research
  • Cell Culture

Quality

Appearance: Clear liquid/no particulates
Sterility Tested: No growth
Endotoxin: <2 EU/mL
pH: 7.0−7.4
Mycoplasma: Negative
Functional Assay: >60% CDX-2+ expression after 4 days of differentiation from iPSC derived DE cells.

Storage and Stability

Upon receipt, store at -20°C. When ready to use, thaw overnight at 2-8°C. Once thawed, mix thoroughly and aliquot into smaller volumes (i.e. 10 mL), use immediately and store at 2-8°C for up to 1 week. Do not re-freeze. Unused aliquots may be stored at -20°C until the expiry date.

Legal Information

3dGRO is a trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Protocols

In Vitro Differentiation of Human iPS Cells Into Colon Organoids in Serum-Free Cell Culture Conditions

Highly characterized cryopreserved human colonic organoids and a step-by-step organoid culture protocol for epithelial intestinal organoid differentiation from iPS cells.

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