MilliporeSigma
All Photos(1)

Documents

SCR727

Millipore

B18R-E3L Plasmid (human codon optimized for B18R and E3L)

To strongly suppress more interferon responses than single B18R to enable more expression of a Simplicon® RNA or regular mRNA(s).

Sign Into View Organizational & Contract Pricing

eCl@ss:
32161000

technique(s)

nucleic acid detection: suitable (RNA)

shipped in

ambient

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
SCR728SCR725SCR726
TagGFP2 Simplicon® Plasmid (E3L) To determine optimal transfection conditions to express the self-replicating RNA of your interest through using Simplicon Cloning Vector (E3L) (Part #:SCR724) in hard-to- transfect somatic or primary cells

SCR725

TagGFP2 Simplicon® Plasmid (E3L)

TagRFP Simplicon® Plasmid (E3L)

SCR726

TagRFP Simplicon® Plasmid (E3L)

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

General description

Simplicon is a novel system to effect immediate high sustained protein expression of the target gene(s) without the risk of integration. The technology employs a self-replicating RNA based on the Venezuelan equine encephalitis (VEE) genome. By nature of a synthetic and self-replicative RNA, interferon (IFN) responses occur at the transfection and replication of Simplicon RNA. The IFN responses reduce the expression and lifetime of Simplicon RNA. Therefore, it is important to suppress the IFN responses in Simplicon System. B18R acts as a decoy receptor for Type I Interferons and inhibit IFN-alpha, beta, and omega and other Type I IFN receptors. When only employing B18R, some IFN-beta response still occurs. The vaccinia virus E3L gene encodes two double-stranded RNA binding proteins that suppressed IFN-beta response and improved the expression of Simplicon RNA at RNA transfection and continuous cell culture. In product B18R-E3L plasmid (human codon optimized for B18R and E3L), B18R and E3L are provided as a polycistronic DNA that can be transcribed into a polycistronic mRNA to be co-transfected with Simplicon RNA(s) for IFN suppression. For continuous expression, B18R protein is needed to supply with B18R-conditioned medium (B18R-CM) or recombinant protein.

Application

B18R-E3L Plasmid (human codon optimized for B18R and E3L) is provided as a polycistronic DNA with both the B18R and E3L genes to more strongly suppress the interferon (IFN) responses that degrades Simplicon RNA(s) or regular mRNA(s). Upon transcription, B18R RNA (E3L) works at single co-transfection with Simplicon RNA or repeated co-transfection with regular mRNA(s). B18R RNA (E3L) can also be used for production of B18R-CM (B18R-conditioned medium).
To strongly suppress more interferon responses than single B18R to enable more expression of a Simplicon RNA or regular mRNA(s).

Storage and Stability

B18R-E3L Plasmid (Cat. # SCR727). One (1) vial containing 10 µL of Plasmid (1 µg/µL). Store at -20 °C

Other Notes

Concentration: Please refer to lot specific datasheet.

Legal Information

SIMPLICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service