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C7930

Sigma-Aldrich

Anti-CD3, T Cell antibody produced in rabbit

whole antiserum

Synonym(s):

Anti-CD3epsilon, Anti-IMD18, Anti-T3E, Anti-TCRE

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About This Item

MDL number:
MFCD00162554
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

conjugate

unconjugated

antibody form

whole antiserum

antibody product type

primary antibodies

clone

polyclonal

mol wt

antigen 20 kDa (bovine and swine)

contains

15 mM sodium azide

species reactivity

rat, canine, mouse, human, Psammomys (sand rat), sheep, feline, pig, chicken, bovine

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200 using enzyme pre-digested, formalin-fixed, paraffin-embedded human tonsil sections
western blot: 1:200 using mouse thymus extract

UniProt accession no.

P07766

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... CD3E(916)
mouse ... Cd3e(12501)
rat ... Cd3e(315609)

General description

CD3 is a 20kDa glycoprotein expressed on surface of all human T lymphocytes. CD3 molecule is encoded by the gene mapped to human chromosome 11q23.3. The CD3 antigen is expressed during thymopoiesis. It is also present in the majority of T-CLL and T-lymphomas and in approximately 70% of T-ALL.

Specificity

The antibody reacts with the proline-rich peptide sequence comprising 13 amino acids in the cytoplasmic domain of the CD3-ε chain. It detects normal, reactive and most neoplastic human T cells in routinely processed enzyme pre-digested, formalin-fixed, paraffin-embedded tissue sections. The antibody also reacts with human tissues fixed with Bouin′s solution and embedded in paraffin. Frozen sections and smears are also suitable for staining with this antibody. The antibody reacts with T cell areas in lymphoid tissues derived from various species.

Immunogen

synthetic human CD3 peptide (residues 156-168 of the CD3-ε chain) coupled to porcine thyroglobulin. Coupling was performed by a modification of the reported method.

Application

Anti-CD3, T Cell antibody produced in rabbit has been used in:
  • immunoblotting
  • immunofluorescence staining
  • immunohistochemistry
  • immunohistochemical analysis

Biochem/physiol Actions

CD3 is a human cell surface glycoprotein complex involved in signal transduction following binding of antigen receptor.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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American Journal of Medical Genetics (1998)
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Neurobiology of disease, 34(1), 63-70 (2009-01-27)
We have found that a small number of purified Th2-biased Abeta-specific T cells are sufficient to provide profound cognitive and pathological benefits in an APP+PS1 mouse model for Alzheimer's disease. Six weeks after receiving T cell infusions, cognitively-impaired mice performed
Fabian P S Yu et al.
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