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C7048

Sigma-Aldrich

Monoclonal Anti-CD3 antibody produced in mouse

clone UCHT-1, purified immunoglobulin, buffered aqueous solution

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Synonym(s):
Monoclonal Anti-CD3
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

UCHT-1, monoclonal

form

buffered aqueous solution

species reactivity

human

technique(s)

flow cytometry: 5 μL using 1 × 106 cells

isotype

IgG1

UniProt accession no.

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

human ... CD3E(916)

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This Item
C7673C7423F0522
antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

clone

UCHT-1, monoclonal

clone

UCHM-1, monoclonal

clone

UCHT-4, monoclonal

clone

UCHT-1, monoclonal

species reactivity

human

species reactivity

human

species reactivity

human

species reactivity

human

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

FITC conjugate

Gene Information

human ... CD3E(916)

Gene Information

human ... CD14(929)

Gene Information

human ... CD8A(925), CD8B(926)

Gene Information

human ... CD3E(916)

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Specificity

Recognizes the CD3 complex. The epitope recognized by clone no. UCHT-1 is expressed on the ε-chain of the CD3 antigen/T cell receptor complex. 3rd Workshop: code no. 126, 208 and 471
4th Workshop: paper no. T3.2

Immunogen

human thymocytes followed by Sezary T cells.

Application

Monoclonal Anti-CD3 antibody produced in mouse is suitable for flow cytometry at a dilution of 5μL using 1 × 106 cells.

Biochem/physiol Actions

The CD3e cytoplasmic domain of the T cell receptor binds to the inner leaflet of the plasma membrane (PM) and is accessible during early stages of T cell activation. It is specific for T lymphocytes, found in Purkinje cells of the cerebellum and gastric parietal cells of humans and other species. The antibodies commonly used to recognize CD3 are directed against the ε-subunit of the T cell receptor. Cytoplasmic cyCD3 expression can only be achieved during the early phase of NK-cell differentiation.

Target description

The CD3 complex (16/20/29/22/25-28 kDa) consists of 5 polypeptide chains associated with each other and with the T cell receptor.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable


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Francesco Dieli et al.
The Journal of infectious diseases, 186(12), 1835-1839 (2002-11-26)
Vgamma9/Vdelta2 T cells can contribute to protective immune response against Mycobacterium tuberculosis, although the extent to which and mechanisms by which they could actually protect against human tuberculosis remain unclear. We have previously reported that Vgamma9/Vdelta2 T cells from tuberculin
Graeme O'Boyle et al.
Proceedings of the National Academy of Sciences of the United States of America, 109(12), 4598-4603 (2012-03-07)
The recruitment of T lymphocytes during diseases such as rheumatoid arthritis is regulated by stimulation of the chemokine receptors expressed by these cells. This study was designed to assess the potential of a CXCR3-specific small-molecule agonist to inhibit the migration
F Dieli et al.
The Journal of infectious diseases, 181(1), 294-301 (1999-12-23)
The alphabeta and gammadelta T cell responses were analyzed in the peripheral blood of children affected by active tuberculosis (TB) and in healthy children who tested positive (PPD+) or negative (PPD-) for purified protein derivative. PPD+ healthy and diseased children
Bernd Schlereth et al.
Cancer research, 65(7), 2882-2889 (2005-04-05)
Bispecific T-cell engager (BiTE) are a class of bispecific single-chain antibodies that can very effectively redirect cytotoxic T cells for killing of tumor target cells. Here, we have assessed the in vivo efficacy of one representative, called bscEp-CAMxCD3, with specificity
Joseph Alroy et al.
Pathology, research and practice, 201(12), 803-808 (2005-11-26)
CD3 antigen, formerly thought to be specific for T lymphocytes, has been identified in Purkinje cells of the cerebellum and gastric parietal cells in several species, including humans. The antibodies commonly used to recognize CD3 are directed against the epsilon-subunit

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