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SRP6539

Sigma-Aldrich

NQO1 human

recombinant, expressed in E. coli, ≥90% (SDS-PAGE)

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Synonym(s):
Azoreductase, DT-Diaphorase (DTD), Dioxin-inducible 1, Disphorase-4 (DIA4), Menadione Reductase, NAD(P)H Dehydrogenase (Quinine) 1 (NQO1), NAD(P)H Quinone Oxireductase, Phylloquinone Reductase, Quinone Oxidoreductase 1, Quinone Reductase 1 (QR1)

biological source

human

recombinant

expressed in E. coli

assay

≥90% (SDS-PAGE)

form

liquid

mol wt

~30 kDa (Human NQO1 aa 1-274 is fused at the C-terminus to a His-tag.)

packaging

pkg of 10 and 50 μg

impurities

<0.1 EU/μg endotoxin (LAL test)

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... NQO1(1728)

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D1315SAB5700008SAB1408944
Sigma-Aldrich

SRP6539

NQO1 human

DT Diaphorase (NQO1) human lyophilized powder, recombinant, expressed in E. coli, &#8805;90% (SDS-PAGE)

D1315

DT Diaphorase (NQO1) human

biological source

human

biological source

-

biological source

rabbit

biological source

mouse

assay

≥90% (SDS-PAGE)

assay

≥90% (SDS-PAGE)

assay

-

assay

-

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

-

recombinant

-

form

liquid

form

lyophilized powder

form

buffered aqueous solution

form

buffered aqueous solution

shipped in

wet ice

shipped in

-

shipped in

wet ice

shipped in

dry ice

General description

NQO1 (NAD(P)H quinone dehydrogenase 1) is an obligate two-electron reductase. It is a cytoplasmic, homodimeric protein, with one FAD molecule per monomer. This gene is localized to human chromosome 16q23. It shows a wide range of tissue expression.

Biochem/physiol Actions

NQO1 (NAD(P)H quinone dehydrogenase 1) uses NADH or NADPH as a reducing cofactor, and is characterized by its inhibition by dicoumarol. It is thought to confer protection against natural and exogenous quinones. It is involved in the conversion of benzene derived quinones to less toxic hydroquinones, and participates in benzene-associated hematotoxicity. It is involved in the reduction and eventual activation of few chemotherapeutic drugs and of environmental carcinogens, such as heterocyclic amines, nitroaromatic compounds, and possibly cigarette smoke particulate. C609T null variant of this gene is linked with breast cancer susceptibility in north Indian patients. NQO1 is up-regulated in serous ovarian carcinoma, and this expression is linked with poor prognosis; hence, this gene has potential as biomarker for the same.

Physical form

0.2 μm-filtered solution in 50 mM Tris-Cl, pH 8.0, containing 1 mM DTT.

pictograms

Exclamation mark

signalword

Warning

hcodes

Hazard Classifications

Eye Irrit. 2

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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High expression of NQO1 is associated with poor prognosis in serous ovarian carcinoma.
Cui X, et al.
BMC Cancer, 15:244 (2015)
The C609T (Pro187Ser) Null Polymorphism of the NQO1 Gene Contributes Significantly to Breast Cancer Susceptibility in North Indian Populations: a Case Control Study.
Yadav P, et al.
Asian Pacific Journal of Cancer Prevention, 17(3), 1215-1219 (2016)
NAD(P)H:quinone acceptor oxidoreductase 1 (NQO1), a multifunctional antioxidant enzyme and exceptionally versatile cytoprotector.
Dinkova-Kostova AT and Talalay P.
Archives of Biochemistry and Biophysics, 501(1), 116-123 (2010)
Xiang Li et al.
Bioorganic & medicinal chemistry, 24(5), 1006-1013 (2016-01-25)
A new series of ortho-naphthoquinone analogs of β-lapachone were designed, synthesized and evaluated. The biological results indicated that most of our compounds were efficient substrates for NQO1. The new scaffold with water-soluble side chain resulted in greater solubility under acidic
Prevalence of the inactivating 609C-->T polymorphism in the NAD(P)H:quinone oxidoreductase (NQO1) gene in patients with primary and therapy-related myeloid leukemia.
Larson RA, et al.
Blood, 94(2), 803-807 (1999)

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