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N5288

Sigma-Aldrich

Anti-NQO1 (C-terminal) antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

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Synonym(s):
Anti-DIA4, Anti-DT-diaphorase, Anti-Diaphorase 4, Anti-NAD (P)H:Menadione oxidoreductase 1, dioxin-inducible 1, Anti-NAD(P)H dehydrogenase, quinone 1, Anti-NMOR1
NACRES:
NA.41

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~28 kDa

species reactivity

mouse, rat, human

concentration

~1 mg/mL

technique(s)

immunoprecipitation (IP): 2-5 μg using whole extract of human HeLa cells
indirect immunofluorescence: 2-5 μg/mL using human HeLa cells
western blot: 0.1-0.2 μg/mL using whole extract of rat NRK cells
western blot: 0.2-0.5 μg/mL using whole extract of mouse 3T3 cells

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... NQO1(1728)
mouse ... Nqo1(18104)
rat ... Nqo1(24314)

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This Item
P0068SAB4200522G6046
Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

biological source

rabbit

biological source

rabbit

biological source

rabbit

biological source

rabbit

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

species reactivity

mouse, rat, human

species reactivity

rat, mouse, human

species reactivity

rat, mouse, human

species reactivity

human, mouse (predicted), rat (predicted)

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General description

NADPH:quinone oxidoreductase (NQO1) is an FAD containing electron reductase that regulates the detoxification of quinones obtained from the phenolic metabolites of benzene. It is a homodimeric protein that can bind either of the reducing cofactors; NADPH or NADH . NQO1 regulates chemoprotection, neuroprotection, antitumor quinones and antioxidative defense mechanisms . NQO1 may be overexpressed in breast, colon, pulmonary and hepatic tumors.
NADPH:quinone oxidoreductase (NQO1, DTdiaphorase) is a 2-electron reductase. NQO1 is a FAD-containing protein that exists as a homodimer. It is ubiquitously expressed.

Specificity

Anti-NQO1 (C-terminal) antibody is specific for rat, mouse, and human NQO1. The immunizing peptide specifically inhibits the detection of NQO1 band by immunoblotting.

Immunogen

Synthetic peptide sequence corresponding to amino acid residues 261-274 of human NQO1, with N-terminal added cysteine conjugated to KLH. The corresponding sequence differs by one amino acid in mouse and rat.
synthetic peptide corresponding to amino acid resudues 261-274 of human NQO1 with N-terminal added cysteine conjugated to KLH. The corresponding sequence differs by one amino acid in mouse and rat.

Application

Anti-NQO1 (C-terminal) antibody may be used in immunological applications such as immunoprecipitation, immunofluorescence and immunoblotting (approximately 28 kDa).
Anti-NQO1 (C-terminal) antibody produced in rabbit has been used in:
  • western blotting
  • immunoprecipitation
  • immunofluorescence

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Biochem/physiol Actions

NADPH:quinone oxidoreductase (NQO1, DTdiaphorase) can bioactivate certain antitumor quinones. NQO1 has a role in antioxidant defense via the generation of antioxidant forms of ubiquinone and vitamin E. High levels of NQO1 gene expression has been observed in liver and lung tumors as compared to normal tissues of the same origin.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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High expression of NQO1 is associated with poor prognosis in serous ovarian carcinoma
Cui X, et al.
BMC Cancer, 15(1), 1-8 (2015)
Neuroprotective effects of urate are mediated by augmenting astrocytic glutathione synthesis and release
Bakshi R, et al.
Neurobiology of Disease, 82, 574-579 (2015)
Christoph Otto et al.
BMC complementary and alternative medicine, 16, 160-160 (2016-06-02)
Fermented wheat germ extract (FWGE) sold under the trade name Avemar exhibits anticancer activity in vitro and in vivo. Its mechanisms of action are divided into antiproliferative and antimetabolic effects. Its influcence on cancer cell metabolism needs further investigation. One
Jennifer M McCracken et al.
Gene expression, 17(3), 187-205 (2017-02-25)
Biological differences exist between strains of laboratory mice, and it is becoming increasingly evident that there are differences between substrains. In the C57BL/6 mouse, the primary substrains are called 6J and 6N. Previous studies have demonstrated that 6J and 6N
NAD(P)H:quinone oxidoreductase 1: role as a superoxide scavenger.
Siegel, D., et al.
J. Mol. Pharmacol., 65(5), 1238-1247 (2004)

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