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D5648

Sigma-Aldrich

Dulbecco′s Modified Eagle′s Medium - high glucose

With 4500 mg/L glucose and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

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Synonym(s):
DME, DMEM
NACRES:
NA.75

form

powder

technique(s)

cell culture | mammalian: suitable

components

phenol red: yes
L-glutamine: yes
NaHCO3: no
HEPES: no
glucose: high
sodium pyruvate: no

shipped in

ambient

storage temp.

2-8°C

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D1152D6171D0422
technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

components

phenol red: yes
L-glutamine: yes
NaHCO3: no
HEPES: no
glucose: high
sodium pyruvate: no

components

sodium pyruvate: no
NaHCO3: no
phenol red: yes
HEPES: 25 mM
L-glutamine: yes
glucose: high

components

sodium pyruvate: no
HEPES: 25 mM
glucose: high
NaHCO3: yes
L-glutamine: no
phenol red: yes

components

glucose: high
phenol red: yes
L-glutamine: no
sodium pyruvate: yes

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

classic media, classical media, FBS, glucose enriched

classic media, classical media, FBS, glucose enriched

classic media, classical media, serum supplement, FBS, HEPES, high glucose, pyridoxine

classic media, classical media, serum supplement, FBS

Application

Dulbecco′s Modified Eagle′s Medium - high glucose has been used as the culture medium for various cell types including the human cervical endocervical epithelial End1/E6E7 cells, human adenocarcinoma epithelial cells (HeLa), human alveolar adenocarcinoma epithelial cells (A549), human osteosarcoma epithelial cells, human diploid cell line , primary hepatocytes and synovial tissue
Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains four-fold concentrations of the amino acids and vitamins. The original formulation contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. Since then, it has been modified in several ways to support primary cultures of mouse and chicken cells, as well as a variety of normal and transformed cells. Each of these media offers a different combination of L-glutamine and sodium pyruvate. Additionally, the glucose levels have been raised to 4500 mg/L, contributing to the name "DMEM/High".

Quantity

Formulated to contain 13.4 grams of powder per liter of medium.

Reconstitution

Supplement with 3.7 g/L sodium bicarbonate.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Articles

Cell culture protocol for passaging and splitting suspension cell lines

Protocols

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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