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D1145

Sigma-Aldrich

Dulbecco′s Modified Eagle′s Medium - high glucose

With 4500 mg/L glucose and sodium bicarbonate, without L-glutamine, sodium pyruvate, and phenol red, liquid, sterile-filtered, suitable for cell culture

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Synonym(s):
DME, DMEM
NACRES:
NA.71

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

L-glutamine: no
sodium pyruvate: no
glucose: high
phenol red: no
NaHCO3: yes
HEPES: no

shipped in

ambient

storage temp.

2-8°C

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This Item
D6171D0422D6546
sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

components

L-glutamine: no
sodium pyruvate: no
glucose: high
phenol red: no
NaHCO3: yes
HEPES: no

components

sodium pyruvate: no
HEPES: 25 mM
glucose: high
NaHCO3: yes
L-glutamine: no
phenol red: yes

components

glucose: high
phenol red: yes
L-glutamine: no
sodium pyruvate: yes

components

NaHCO3: yes
phenol red: yes
sodium pyruvate: yes
HEPES: no
L-glutamine: no
glucose: high

form

liquid

form

liquid

form

liquid

form

liquid

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

Application

Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains four-fold concentrations of the amino acids and vitamins. The original formulation contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. Since then, it has been modified in several ways to support primary cultures of mouse and chicken cells, as well as a variety of normal and transformed cells. Each of these media offers a different combination of L-glutamine and sodium pyruvate. Additionally, the glucose levels have been raised to 4500 mg/L, contributing to the name "DMEM/High".

Reconstitution

Supplement with 0.584 g/L L-glutamine.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Ştefan Ţălu et al.
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