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D5546

Sigma-Aldrich

Dulbecco′s Modified Eagle′s Medium - low glucose

With 1000 mg/L glucose, and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

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Synonym(s):
DME, DMEM
MDL number:
NACRES:
NA.75

Quality Level

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

NaHCO3: yes
phenol red: yes
glucose: low
HEPES: no
sodium pyruvate: yes
L-glutamine: no

shipped in

ambient

storage temp.

2-8°C

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This Item
D5921D2429D9443
components

NaHCO3: yes
phenol red: yes
glucose: low
HEPES: no
sodium pyruvate: yes
L-glutamine: no

components

phenol red: no
sodium pyruvate: no
glucose: low
HEPES: no
L-glutamine: no
NaHCO3: yes

components

NaHCO3: no
sodium pyruvate: yes
phenol red: yes
glucose: low
L-glutamine: no
HEPES: no

components

NaHCO3: 3.7 g/L
sodium pyruvate: no
glucose: 1.0 g/L
L-glutamine: 0.584 g/L
phenol red: no
HEPES: no

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

form

liquid

form

liquid

form

liquid

form

liquid

impurities

endotoxin, tested

impurities

endotoxin, tested

impurities

endotoxin, tested

impurities

endotoxin, tested

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

General description

Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains a higher concentration of amino acids, vitamins, and additional supplementary components. The original DMEM formula, first reported for culturing embryonic mouse cells, contained 1,000 mg/L of glucose. An alteration with 4,500 mg/L glucose is optimal in cultivating certain cell types. This modification has been shown to improve the stability of the medium. 

Application

Dulbecco′s Modified Eagle′s Medium - low glucose has been used to culture:

  • human osteosarcoma cells and HeLa cells
  • primary human mesenchymal stromal cells (hBMSCs)
  • human embryonic kidney cell lines

Reconstitution

Supplement with 0.584 g/L glutamine.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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