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B1522

SAFC

Basal Medium Eagle

With Earle′s salts and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

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Synonym(s):
BME
NACRES:
NA.75

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

L-glutamine: no
NaHCO3: yes
Earle’s salts (5% CO2): yes

shipped in

ambient

storage temp.

2-8°C

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1 of 4

This Item
M0268B963851416C
vibrant-m

B1522

Basal Medium Eagle

vibrant-m

M0268

Minimum Essential Medium Eagle

vibrant-m

B9638

Basal Medium Eagle

vibrant-m

51416C

Minimum Essential Medium

components

L-glutamine: no
NaHCO3: yes
Earle’s salts (5% CO2): yes

components

HEPES: no
phenol red: yes
Earle’s salts (5% CO2): yes
NaHCO3: no
L-glutamine: yes
sodium pyruvate: no

components

phenol red: 0.011 g/L
NaHCO3: no
L-glutamine: 0.292 g/L
Earle’s salts (5% CO2): yes

components

Earle’s salts (5% CO2): yes
L-glutamine: no

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

form

liquid

form

powder

form

powder

form

liquid

Quality Level

400

Quality Level

500

Quality Level

500

Quality Level

400

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

General description

Eagle′s Basal Medium (BME) is one of the most used synthetic cell culture media. The tissue culture association recommends that the term "Eagle′s Basal Medium" be used only to refer to the formula developed to support HeLa cells. BME has previously been used in monolayer culture studies to assess the growth response of normal (WI-38) and transformed (mouse and HeLa) cells. BME is the precursor of Dulbecco′s Modified Eagle′s medium (DME) and Eagle′s Minimum Essential Medium (MEM).

Application

Basal Medium Eagle has been used:
  • as a component in the culture medium for cochlear organotypic cultures and salicylate treatment
  • to maintain cerebellar granule neurons (CGNs) to determine if there is a system that regulates the distribution and/or group dynamics of axonal mitochondria
  • in the preparation of cortical slices, serum-free medium, and slice culture medium for slice overlay assay to study the influence of extracellular signals on neuronal development

Reconstitution

Supplement with 0.292 g/L L-glutamine.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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