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SAB4700040

Sigma-Aldrich

Monoclonal Anti-CD3 antibody produced in mouse

clone OKT3, purified immunoglobulin, buffered aqueous solution

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Synonym(s):
Mouse Anti-T-cell surface antigen T3/Leu-4 epsilon chain, Mouse Monoclonal Anti-T3E
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

OKT3, monoclonal

form

buffered aqueous solution

species reactivity

human

concentration

1 mg/mL

technique(s)

flow cytometry: suitable

isotype

IgG2a

NCBI accession no.

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

human ... CD3(916)

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This Item
SAB4700050C7048SAB4700044
Gene Information

human ... CD3(916)

Gene Information

human ... CD3(916)

Gene Information

human ... CD3E(916)

Gene Information

human ... CD3(916)

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

species reactivity

human

species reactivity

human, mouse

species reactivity

human

species reactivity

human

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

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General description

The CD3 gene is mapped to human chromosome 11q23.3. The encoded protein exists in three isoforms, CD3ε, CD3γ and CD3δ and each contains an N-terminal extracellular domain, a transmembrane segment and a cytoplasmic domain. CD3 is a 20kDa glycoprotein expressed on surface of all human T lymphocytes.
The mouse monoclonal antibody OKT3 recognizes the CD3 antigen of the TCR/CD3 complex on mature human T cells. This antibody, also known as Orthoclone OKT3 or Muromonab-CD3, has been extensively used as a drug for therapy of acute, glucocorticoid resistant rejection of allogenic renal, heart and liver transplants. It has also been investigated for use in treating T-cell acute lymphoblastic leukemia.

Application

Monoclonal Anti-CD3 antibody produced in mouse has been used in proliferation assay.
The reagent is designed for Flow Cytometry analysis. Suggested working dilution is 1 μg/mL of sample. Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator.

Biochem/physiol Actions

T-cell receptor-CD3 complex including CD3ε, CD3γ and CD3δ, plays a vital role in inducing early metabolic events that lead to T cell activation. Mutation in the CD3ε has been observed in T-B+ NK+ severe combined immunodeficiency (SCID) patients.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Physical form

Solution in phosphate buffered saline, pH 7.4, with 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable


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