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C7831

Sigma-Aldrich

Anti-CD28 antibody, Mouse monoclonal

clone CD28.2, purified from hybridoma cell culture

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Synonym(s):
Monoclonal Anti-CD28

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

CD28.2, monoclonal

form

buffered aqueous solution

mol wt

antigen 44 kDa

species reactivity

human, rhesus monkey

technique(s)

flow cytometry: 5 μL using 1 × 106 cells
immunocytochemistry: suitable
immunohistochemistry (frozen sections): suitable

isotype

IgG1

UniProt accession no.

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

human ... CD28(940)

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General description

CD28 (Cluster of differentiation 28) is a glycosylated membrane protein. The protein exists as a homodimer. CD28 is encoded by the gene mapped to human chromosome 2q33. The encoded protein is a co-stimulatory receptor expressed on naive T-cells. CD28 is characterized with a short cytoplasmic tail and two proline-rich motifs.

Specificity

Recognizes the CD28 (44 kDa) human cell surface disulfide-linked, homodimeric glycoprotein. The antibody reacts with human CD28-transfected murine T-cell hybridoma, PHA-activated blasts and IL-6 dependent plasmacytoma lines. It cross reacts with rhesus monkey PBL. The antibody blocks CD28/B7 (CD80) mediated cell-cell adhesion. It binds to the CDR domain in a CDR1 dependent manner. It enhances CD3 monoclonal antibody-induced T-cell proliferation. May be used in adhesion and co-stimulation studies.
5th Workshop: code no. CD28.05.

Immunogen

human CD28 transfected murine T cell hybridoma.

Application

Monoclonal Anti-CD28 antibody produced in mouse is suitable for the following applications:
  • Flow cytometry at a dilution of 5μL using 1 × 106 cells
  • Immunocytochemistry
  • Immunohistochemistry (frozen sections)
The antibody has been used for development of an assay to identify small molecules antagonists of CD28/CD86 binding.

Biochem/physiol Actions

CD28 (Cluster of differentiation 28) is a protein expressed on T-cells that provide co-stimulatory signals required for T-cell activation, survival and differentiation into effector T cells. CD28 is also expressed on multiple myeloma (MM) cells and is a key mediator of MM survival and apoptotic resistance. CD28 pro-survival signaling is dependent upon downstream activation of phosphatidylinositol 3-kinase (PI3K)/AKT (RAC-alpha serine/threonine-protein kinase), inactivation of the transcription factor FoxO3a and decreased expression of the pro-apoptotic molecule BIM. CD28 plays an important role as a mediator of MM survival during stress and can be targeted to overcome chemotherapy resistance. Inflammatory CD28- T-cells are found to be accumulated in livers of patients with primary sclerosing cholangitis (PSC) and are localized around bile ducts. Autoantibodies against CD28 are found in patients with autoimmune and atopic diseases.

Target description

CD28 is expressed on thymocytes, peripheral lineage cell subsets, activated B cells and plasma cells. The antigen is involved in adhesion between T and B lymphocytes through the B7/BB-1 ligand expressed on activated B cells. CD28 co-stimulates T-cells activated via the CD3 T-cell receptor and CD2 pathways and induces their proliferation.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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CD28 Deficiency Enhances Type I IFN Production by Murine Plasmacytoid Dendritic Cells.
Macal M, et.al.
Journal of Immunology, 196, 1900-1909 (2016)
Harjit Dadi et al.
The Journal of allergy and clinical immunology, 141(5), 1818-1830 (2017-08-23)
Combined immunodeficiency (CID) is a T-cell defect frequently presenting with recurrent infections, as well as associated immune dysregulation manifesting as autoimmunity or allergic inflammation. We sought to identify the genetic aberration in 4 related patients with CID, early-onset asthma, eczema
Development of a CD28/CD86 (B7-2) Binding Assay for High Throughput Screening by Homogeneous Time-Resolved Fluorescence.
Mellor G W, et al.
Journal of Biomolecular Screening, 3, 91-99 (1998)
Evaggelia Liaskou et al.
Gastroenterology, 147(1), 221-232 (2014-04-15)
T-cell-mediated biliary injury is a feature of primary sclerosing cholangitis (PSC). We studied the roles of CD28(-) T cells in PSC and their regulation by vitamin D. Peripheral and liver-infiltrating mononuclear cells were isolated from blood or fresh liver tissue.
A Aruffo et al.
Proceedings of the National Academy of Sciences of the United States of America, 84(23), 8573-8577 (1987-12-01)
CD28 (Tp44) is a human T-cell-specific homodimer surface protein that may participate in T-cell activation. We have isolated a cDNA clone encoding CD28 by a simple and highly efficient cloning strategy based on transient expression in COS cells. Central to

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