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SCT045

Sigma-Aldrich

BioTracker ATP-Red Live Cell Dye

Live cell imaging dye for cellular adenosine triphosphate (ATP) localized to mitochondria used to detect metabolic activity, cell proliferation and overall cell health.

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Synonym(s):
Live cell imaging probe

Assay

≥98% (H-NMR)
≥98% (HPLC)
≥98% (LC/MS)
≥98% (elemental analysis)

technique(s)

cell based assay: suitable

detection method

fluorometric

shipped in

ambient

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This Item
SCT137SCT044SCT135
technique(s)

cell based assay: suitable

technique(s)

cell based assay: suitable

technique(s)

cell based assay: suitable

technique(s)

cell based assay: suitable

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

detection method

fluorometric

detection method

fluorometric

detection method

fluorometric

detection method

fluorometric

General description

Adenosine triphosphate (ATP) is the primary energy source for all cellular processes. ATP also functions as a signaling molecule for regulating cell movement, neurotransmission and ion channel functions. ATP is localized in mitochondria, where cellular respiration occurs. ATP levels can be used to measure cell proliferation and cell cycle dynamics.

The BioTracker ATP-Red dye is a live cell red fluorescent imaging probe for adenosine triphosphate (ATP). The probe targets ATP specifically in the mitochondria of living cells. The probe shows no cross reactivity to numerous analytes including: Zn2+, Mg2+, Ca2+, Na2+, K+, GSH, HOCL, H2O2, arabinose, galactose, glucose, fructose, ribose, sorbose, sucrose, xylose, heparin, AMP, ADP, CMP, CDP, CTP, UMP, UDP, UTP, GMP, GDP or GTP. The probe is non-fluorescent when forming a closed ring structure. In the presence of the negatively charged ATP, the covalent bonds between boron and ribose is broken and the ring opens producing fluorescence.

Spectral Properties
Absorbance: 510nm
Emission: 570nm

Application

Live cell fluorescent imaging
Research Category
Cell Imaging
Research Sub Category
Live Cell Dye

Quality

Purity: ≥ 98% confirmed by HNMR, LC-MS and HPLC and elemental analysis
Molar Mass: 561.48 g/mol

Physical form

Lyophilized

Storage and Stability

Store BioTracker ATP-Red Live Cell Dye at -20°C, desiccate and protect from light
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Lu Wang et al.
Angewandte Chemie (International ed. in English), 55(5), 1773-1776 (2015-12-18)
Adenosine triphosphate (ATP), commonly produced in mitochondria, is required by almost all the living organisms; thus fluorescent probes for monitoring mitochondrial ATP levels fluctuation are essential and highly desired. Herein, we report a multisite-binding switchable fluorescent probe, ATP-Red 1, which selectively

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