SCT109
BioTracker 400 Blue Cytoplasmic Membrane Dye
Live cell imaging lipophilic carbocyanine membrane dye suitable for long-term fluorescent cell labeling and cell tracking studies.
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Live cell imaging probe, Live cell membrane dye
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technique(s)
cell based assay: suitable
detection method
fluorometric
Related Categories
General description
Lipophilic carbocyanine dyes are widely used for labeling neurons in tissues by retrograde labeling, and to label membranes in a wide variety of cell types. The dyes are weakly fluorescent in aqueous phase, but become highly fluorescent in lipid bilayers. Staining is highly stable with low toxicity and very little dye transfer in between cells, making the dyes suitable for long-term cell labeling and tracking studies. When live cells are stained, the dyes label plasma membranes and also are taken up into endocytic compartments. Cells can be fixed either before or after staining, although permeabilization affects the staining pattern.
Unlike PKH dyes, BioTracker Cytoplasmic Membrane Dyes do not require a complicated hypoosmotic labeling protocol. They are ready-to-use dye delivery solutions that can be added directly to normal culture media to label suspended or adherent cells in culture.
Spectral Properties
Absorbance: 366nm
Emission: 441nm
Unlike PKH dyes, BioTracker Cytoplasmic Membrane Dyes do not require a complicated hypoosmotic labeling protocol. They are ready-to-use dye delivery solutions that can be added directly to normal culture media to label suspended or adherent cells in culture.
Spectral Properties
Absorbance: 366nm
Emission: 441nm
Application
Live cell fluorescent imaging
Live cell imaging lipophilic carbocyanine membrane dye suitable for long-term fluorescent cell labeling and cell tracking studies.
Research Category
Cell Imaging
Cell Imaging
Research Sub Category
Live Cell Dye
Live Cell Dye
Components
1. 1 vial of 250µL Cell Labeling Solution (CS224586)
2. 1 vial of 250µL Loading Buffer (CS224587)
2. 1 vial of 250µL Loading Buffer (CS224587)
Quality
Spectral Properties
Absorbance: 366nm
Emission: 441nm
Absorbance: 366nm
Emission: 441nm
Physical form
Liquid
Storage and Stability
Store BioTracker 400 Blue Cytoplasmic Membrane Dye at room temp. Protect From Light.
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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