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SCT044

Sigma-Aldrich

BioTracker LYSO-TP Live Cell Dye

Live cell imaging dye for hypochlorous acid (HOCl) targeted to cellular lysosomes used to detect phagocytosis, inflammation and cellular apoptosis.

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Synonym(s):
Live cell imaging probe

assay

≥98% (H-NMR)
≥98% (HPLC)
≥98% (LC/MS)
≥98% (elemental analysis)

technique(s)

cell based assay: suitable

detection method

fluorometric

shipped in

ambient

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SCT044

BioTracker LYSO-TP Live Cell Dye

vibrant-m

SCT043

BioTracker TP-HOCL1 Live Cell Dye

vibrant-m

SCT046

BioTracker CyP AP Live Cell Dye

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

assay

≥98% (H-NMR), ≥98% (LC/MS), ≥98% (HPLC), ≥98% (elemental analysis)

assay

≥98% (H-NMR), ≥98% (HPLC), ≥98% (LC/MS), ≥98% (elemental analysis)

assay

≥98% (H-NMR), ≥98% (HPLC), ≥98% (LC/MS), ≥98% (elemental analysis)

assay

-

detection method

fluorometric

detection method

fluorometric

detection method

fluorometric

detection method

fluorometric

General description

Hypochlorous acid (HOCl) is a highly potent reactive oxygen species (ROS) and helps eliminate pathogens in the innate immune system. Mounting evidence indicates that intracellular HOCl plays additional important roles in regulating inflammation and cellular apoptosis. Subcellular detection of HOCL is currently limited due to low concentration, strong oxidization, and short lifespan of the analyte.

The BioTracker LYSO-TP dye is a live cell two-photon green fluorescent turn-on imaging probe for HOCL. The probe targets HOCL specifically in the lysosomes of cells. The probe exhibit fast response times, good selectivity, and high sensitivity towards hypochlorous acid in living cells. Acedan was chosen as the fluorescence reporting group due to its excellent photophysical properties resulting from the typical push−pull (amineketone) structure. Reaction of the probe with HOCl, which deprotects the oxathiolane/mercaptal group to reveal the ketone, would lead to fluorescence enhancement. To monitor subcellular HOCL levels, morpholine was introduced at a long distance from the reaction site to ensure the probes intracellular localization.

Spectral Properties
Absorbance: 375nm
Emission: 500nm

Application

Live cell fluorescent imaging
Research Category
Cell Imaging
Research Sub Category
Live Cell Dye

Quality

Purity: ≥ 98% confirmed by HNMR, LC-MS and HPLC and elemental analysis
Molar Mass: 357.49 g/mol

Physical form

Lyophilized

Storage and Stability

Store BioTracker LYSO-TP Live Cell Dye at -20°C, desiccate and protect from light
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Lin Yuan et al.
Journal of the American Chemical Society, 137(18), 5930-5938 (2015-04-24)
Hypochlorous acid (HOCl), as a highly potent oxidant, is well-known as a key "killer" for pathogens in the innate immune system. Recently, mounting evidence indicates that intracellular HOCl plays additional important roles in regulating inflammation and cellular apoptosis. However, the

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