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SCT244

Sigma-Aldrich

BioTracker Dual-Color Mitochondria-Lysosome Live Cell Dye

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Synonym(s):
Live Cell Imaging Probe

mol wt

calculated mol wt 586.46 kDa

Quality Level

packaging

vial of 1 mg

manufacturer/tradename

Millipore

storage condition

protect from light

growth mode

N/A

technique(s)

cell based assay: suitable
cell culture | stem cell: suitable
flow cytometry: suitable

detection method

fluorometric

shipped in

dry ice

storage temp.

−20°C (−15°C to −25°C)

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SCT084SCT083SCT137
technique(s)

cell based assay: suitable, flow cytometry: suitable, cell culture | stem cell: suitable

technique(s)

cell based assay: suitable, flow cytometry: suitable

technique(s)

cell based assay: suitable, flow cytometry: suitable

technique(s)

cell based assay: suitable

storage temp.

−20°C (−15°C to −25°C)

storage temp.

−20°C

storage temp.

−20°C

storage temp.

-

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

ambient

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

-

manufacturer/tradename

Millipore

manufacturer/tradename

Millipore

manufacturer/tradename

Millipore

manufacturer/tradename

-

General description

The BioTracker Dual-Color Mitochondria-Lysosome Live Cell Dye (Coupa) is a fluorescent probe used to detect mitochondria-lysosome interactions (Chen, 2020).


Mitochondrial-lysosomal interactions are fundamental to the processes of cellular homeostasis. Mitochondria and lysosomes have unique interactions which involve transfer of metabolites. Some disease pathogenesis can be linked to this important subcellular interaction (Chen, 2020). This BioTracker live cell probe is a unique cell-permeable and viscosity-responsive molecular probe that is capable of monitoring mitochondrial and lysosomal interactions in living cells. Through fluorescence conversion, this BioTracker dual color probe can simultaneously tag mitochondria with blue fluorescence and lysosomes with red fluorescence.


Spectral Properties


Fluorescence images obtained by λex = 405 nm and emission at 450 nm (Mitochondria) and λex = 561nm and emission at 590nm (Lysosome)


Reference
Chen, Qixin, et al. A dual-labeling probe to track functional mitochondria–lysosome interactions in live cells." Nature Communications 11.1 (2020): 1-10."

Application

Purity > 98% confirmed by HPLC. Identification confirmed by HNMR, LC-MS, and elemental analysis.

Features and Benefits

Dual color (blue and red) small molecule probe for visualizing mitochondria-lysosome interactions during mitophagy.

Physical form

Lyophilized. Brown solid.

Storage and Stability

Store at -20°C, dessicated and protected from light. Note: Centrifuge vial briefly to collect contents at bottom of vial before opening

Other Notes

This product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@emdmillipore.com.

Legal Information

BioTracker is a trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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