Skip to Content
MilliporeSigma
All Photos(1)

Key Documents

WBKLS

Millipore

Immobilon Western Chemiluminescent HRP Substrate

Immobilon Western HRP Substrate provides high sensitivity for Chemiluminescent detection in western or dot/slot/spot blotting applications on both PVDF and nitrocellulose membranes, and is compatible with all commonly used buffers and blocking reagents.

Synonym(s):

HRP substrate, chemiluminescent detection substrate, detection substrate

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

UNSPSC Code:
41116010
eCl@ss:
42029053
NACRES:
NB.22

conjugate

peroxidase conjugate

Quality Level

200

packaging

pkg of 250 mL Luminol
pkg of 250 mL Peroxide solution

manufacturer/tradename

Immobilon®

technique(s)

western blot: suitable

membrane area

5000 cm2 , membrane coverage

detection method

chemiluminescent

shipped in

ambient

General description

Immobilon Western HRP Substrate is compatible with all commonly used buffers and blocking reagents and provides high sensitivity for chemiluminescent detection in western or dot/slot/spot blotting applications on both PVDF and nitrocellulose membranes.

Application

Immobilon Western Chemiluminescent HRP Substrate is suitable for western blotting.

Legal Information

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Nicolas Bidère et al.
Nature, 458(7234), 92-96 (2009-01-02)
The transcription factor NF-kappaB is required for lymphocyte activation and proliferation as well as the survival of certain lymphoma types. Antigen receptor stimulation assembles an NF-kappaB activating platform containing the scaffold protein CARMA1 (also called CARD11), the adaptor BCL10 and
Elizabeth I Vink et al.
Virology, 422(2), 317-325 (2011-11-30)
The adenovirus E4-ORF3 protein promotes viral replication by relocalizing cellular proteins into nuclear track structures, interfering with potential anti-viral activities. E4-ORF3 targets transcriptional intermediary factor 1 alpha (TIF1α), but not homologous TIF1β. Here, we introduce TIF1γ as a novel E4-ORF3-interacting
Reinhard David et al.
The European journal of neuroscience, 31(6), 978-993 (2010-04-10)
Nicotinic acetylcholine receptors (nAChRs) mediate fast synaptic transmission in ganglia of the autonomic nervous system. Here, we determined the subunit composition of hetero-pentameric nAChRs in the mouse superior cervical ganglion (SCG), the function of distinct receptors (obtained by deletions of
Novel irreversible epidermal growth factor receptor inhibitors by chemical modulation of the cysteine-trap portion.
Carmi, Caterina, et al.
Journal of medicinal chemistry, 53, 2038-2050 (2010)

Articles

Dot/Slot (Filtration) Blotting

The possible causes and potential remedies for challenges encountered dot/slot blotting, which uses vacuum filtration to transfer protein onto a microporous membrane.

Western Blotting: Why Are Observed and Calculated Molecular Weights Different?

A discussion of factors affecting observed vs. calculated molecular weight in gel electrophoresis and western blotting.

Protocols

Stripping and Reprobing Western Blotting Membranes

This page shows and discusses three protocols for stripping and reprobing a western blot membrane.

Cell Lysis and Protein Extraction for Western Blotting

Protocol for sample preparation for cell lysis and efficient protein extraction from cultured tissues and cells for subsequent Western blotting.

Related Content

Protein Research Guide

Find protein research tools to prepare, isolate, and analyze proteins. Organized by how to extract, protect, purify, enrich, modify, and quantify proteins.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service