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11089161001

Roche

Streptavidin-AP conjugate

solution, pkg of 1 mL (1,000U)

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Synonym(s):
streptavidin

form

solution

Quality Level

packaging

pkg of 1 mL (1,000U)

manufacturer/tradename

Roche

storage temp.

2-8°C

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S3887S289011093266910
Streptavidin-AP conjugate solution, pkg of 1 mL (1,000U)

11089161001

Streptavidin-AP conjugate

form

solution

form

lyophilized powder

form

lyophilized powder

form

solution

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

packaging

pkg of 1 mL (1,000U)

packaging

-

packaging

-

packaging

pkg of 200 μL (150U)

manufacturer/tradename

Roche

manufacturer/tradename

-

manufacturer/tradename

-

manufacturer/tradename

Roche

General description

Streptavidin is covalently coupled to alkaline phosphatase (AP) for the preparation of streptavidin-AP-conjugate.

Alkaline Phosphatase
Alkaline Phosphatase is often used as a label in enzyme immunoassays. AP converts the sensitive substrate X-phosphate/NBT into a stable, water soluble product.

Application

Streptavidin-AP has been used to detect biotinylated compounds using:
  • ELISA
  • Immunohistocytochemistry
  • Western blot

Physical form

Solution, stablilized in 50 mM Triethanolamine buffer, 3 M NaCl, 1 mM MgCl2, 0.1 mM ZnCl2, 10 mg/ml bovine serum albumin, pH 7.6.

Preparation Note

Working solution: 50 - 1,000 mU/ml (i.e., stock solution can be diluted). Dilute the conjugate stock solution in 100 mM Tris-HCl, 150 mM NaCl, pH 7.5.

Working concentration: Working concentration of conjugate will depend on the application and substrate. Indicated concentrations should be taken as a guideline.

ELISA: 50 - 200 mU/ml (1:5000 - 1:20,000 dilution)
Immunohisto/cytochemistry: 250 - 1,000 mU/ml (1:1000 - 1:4000 dilution)
Western blot: 200 - 500 mU/ml (1:2000 - 1:5000 dilution)

Stability: The concentrated stock solution is stable when stored at 2 to 8°C until the expiration date printed on the label. Do not freeze!

Other Notes

For life science research only. Not for use in diagnostic procedures.

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Warning

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Hazard Classifications

Skin Sens. 1

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

No data available

flash_point_c

No data available


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