11333054001

Digoxigenin-3-O-methylcarbonyl-ε-aminocaproic acid-N-hydroxysuccinimide ester

>90% (elementary analysis), pkg of 5 mg, powder, pH range 2-13

• Synonym(s): Digoxigenin NHS-ester, dig-nhs-ester, ε-(Digoxigenin-3-0-acetamido)caproic acid N-hydroxysuccinimide ester, DIG NHS
• Empirical Formula (Hill Notation): C₃₅H₅₀N₂O₁₀
• CAS Number: 129273-26-3
• Molecular Weight: 658.78
• MDL number: MFCD02093394
• UNSPSC Code: 41105600
• PubChem Substance ID: 329749387

Properties

• assay: >90% (elementary analysis)
• form: powder
• mol wt: M_r 658.8
• packaging: pkg of 5 mg
• manufacturer/tradename: Roche
• pH range: 2-13
• solubility: DMF: soluble; DMSO: soluble; ethanol: soluble
• storage temp.: −20°C
• SMILES string: [H][C@]12CC[C@]3([H])[C@]([H])(C[C@@H](O)[C@]4(C)[C@H](CC[C@]34O)C5=CC(=O)OC5)[C@@]1(C)CC[C@@H](C2)OCC(=O)NCCCCCC(=O)ON6C(=O)CCC6=O
• InChI: 1S/C35H50N2O10/c1-33-13-11-23(45-20-28(39)36-15-5-3-4-6-31(42)47-37-29(40)9-10-30(37)41)17-22(33)7-8-25-26(33)18-27(38)34(2)24(12-14-35(25,34)44)21-16-32(43)46-19-21/h16,22-27,38,44H,3-15,17-20H2,1-2H3,(H,36,39)/t22-,23+,24-,25-,26+,27-,33+,34+,35+/m1/s1
• InChI key: KHNDABJZSPPYLE-FUGFVFQCSA-N

Description

General description

Chemical reagent for the labeling of proteins and oligonucleotides. One reactive group is able to interact with NH₂-groups (of proteins or oligonucleotides), another reactive group (Digoxigenin) reacts with Anti-Digoxigenin and thus can be detected.

Application

Digoxigenin-3-O-methylcarbonyl-ε-aminocaproic acid-N-hydroxysuccinimide ester has been used to label the sequence (III) for the linker molecule sequence (VIII).

Biochem/physiol Actions

Digoxigenin, a non-radioactive immune-tag is mainly used for labeling proteins and in situ hybridization.

Principle

The activated ester reacts with amino groups under mild conditions. The digoxigenin moiety can be introduced into proteins or 5′-amino substituted oligonucleotides.

Preparation Note

Working concentration: 1 to 5 mM
Working solution: Preparation of working solution

Under stirring 0.327 mg digoxigenin-3-O-methylcarbonyl- ε-aminocaproic acid -N-hydroxysuccinimide ester is dissolved in 8.18 μl DMSO or ethanol.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Safety Information

• pictograms: GHS06,GHS08,GHS09
• signalword: Danger
• hcodes: H300,H319,H331,H373,H410
• pcodes: P260 - P264 - P273 - P301 + P310 + P330 - P391 - P403 + P233
• Hazard Classifications: Acute Tox. 2 Oral - Acute Tox. 3 Inhalation - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Irrit. 2 - STOT RE 2
• Storage Class: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable