11684817910
Roche
In Situ Cell Death Detection Kit, POD
sufficient for ≤50 tests
Synonym(s):
cell death
About This Item
Recommended Products
usage
sufficient for ≤50 tests
Quality Level
manufacturer/tradename
Roche
technique(s)
immunohistochemistry: suitable
storage temp.
−20°C
Related Categories
General description
Application
- Examples are: Detection of individual apoptotic cells in frozen, paraffin-embedded and formalin-fixed tissue sections in basic research and routine pathology
- Determination of sensitivity of malignant cells to drug-induced apoptosis in cancer research and clinical oncology
- Typing of cells undergoing cell death in heterogeneous populations by double staining procedures
Features and Benefits
Fast: The use of fluorescein-dUTP allows analysis of the samples directly after the TUNEL reaction, but before the addition of the secondary detection system.
Convenient: The direct labeling procedure using fluorescein-dUTP allows verification of the efficiency of the TUNEL reaction during the assay procedure.
Accurate: Identification of apoptosis at a molecular level (DNA-strand breaks) and identification of cells at the very early stages of apoptosis.
Flexible: No substrate included; provides the opportunity to select the staining procedure of choice.
Packaging
Principle
Apoptotic cells are fixed and permeabilized. Subsequently, the cells are incubated with the TUNEL reaction mixture that contains TdT and fluorescein-dUTP. During this incubation period, TdT catalyzes the addition of fluorescein-dUTP at free 3′-OH groups in single- and double-stranded DNA. After washing, the label incorporated at the damaged sites of the DNA is marked by an anti-fluorescein antibody conjugated with the reporter enzyme peroxidase. After washing to remove unbound enzyme conjugate, the POD retained in the immune complex is visualized by a substrate reaction.
Preparation Note
The TUNEL reaction mixture should be prepared immediately before use and should not be stored. Keep TUNEL reaction mixture on ice until use.
Converter-peroxidase
Once thawed the Converter-peroxidase solution should be stored at 2 to 8 °C (maximum stability 6 months).
Note: Do not freeze.
Sample material: Cytospin and cell smear preparations, adherent cells grown on slides, and frozen and paraffin-embedded tissue sections.
Kit Components Only
- Enzyme Solution (TdT)
- Label Solution (fluorescein-dUTP)
- Converter Peroxidase (anti-fluorescein antibody-peroxidase) ready-to-use
signalword
Danger
hcodes
Hazard Classifications
Aquatic Chronic 2 - Carc. 1B Inhalation - Skin Sens. 1
wgk_germany
WGK 3
flash_point_f
does not flash
flash_point_c
does not flash
Certificates of Analysis (COA)
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Cellular apoptosis assays to detect programmed cell death using Annexin V, Caspase and TUNEL DNA fragmentation assays.
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