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APT110

Sigma-Aldrich

Apo-Direct TUNEL Assay Kit

The APO-DIRECT Kit is a single-step staining method for labeling DNA breaks to detect apoptotic cells by flow cytometry.

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eCl@ss:
32161000

Quality Level

species reactivity

vertebrates

manufacturer/tradename

ApoDIRECT
Chemicon®

technique(s)

flow cytometry: suitable

detection method

fluorometric

shipped in

wet ice

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APOAFS7111S7110
Sigma-Aldrich

APT110

Apo-Direct TUNEL Assay Kit

species reactivity

vertebrates

species reactivity

-

species reactivity

-

species reactivity

-

manufacturer/tradename

ApoDIRECT

manufacturer/tradename

-

manufacturer/tradename

ApopTag, Chemicon®

manufacturer/tradename

ApopTag, Chemicon®

detection method

fluorometric

detection method

fluorometric

detection method

fluorometric

detection method

fluorometric

shipped in

wet ice

shipped in

wet ice

shipped in

dry ice

shipped in

dry ice

Quality Level

100

Quality Level

300

Quality Level

100

Quality Level

100

General description

The CHEMICON APO-DIRECT Kit is a single-step staining method for labeling DNA breaks to detect apoptotic cells by flow cytometry (Li et al. 1995). The kit contains all the reagents required for measuring apoptosis in cells including positive and negative control cells; washing, reaction, and rinsing buffers; and propidium iodide/RNase A solution for counter staining the total DNA.

Application

Research Category
Apoptosis & Cancer
The APO-DIRECT Kit is a single-step staining method for labeling DNA breaks to detect apoptotic cells by flow cytometry.

Components

The following components are included with the APO-DIRECT Kit. Reagent bottles have color coded caps to aid in their identification. Sufficient reagents are provided to process 50 cell suspensions including control cells of approximately 1 x 106 cells per suspension for flow cytometry analysis. The control cells have been fixed as described (under the heading cell fixation procedure) and are in 70% (v/v) ethanol.

Positive Control Cells,Brown cap

Negative Control Cells, Natural cap

Wash Buffer, Blue cap

Reaction Buffer, Green cap

TdT Enzyme, Yellow cap

F-dUTP, Orange cap

Rinsing Buffer, Red cap

PI/RNase Staining Buffer, Amber bottle

Storage and Stability

Precautions and Warnings:

1. The components of this kit are for Research Use only and are not intended for diagnostic procedures.

2. Positive and negative control cells contain 70% (v/v) ethanol as a preservative; wash and reaction buffer contain sodium cacodylate (dimethylarsinic) as a buffer; rinsing and PI/RNase staining buffer contain 0.05% (w/v) sodium azide as a preservative. These materials are harmful if swallowed; avoid areas of contact immediately. See Material Safety Data Sheets.

3. TdT Enzyme will not freeze at -20oC, because it is in 50% (v/v) glycerol solution. Upon warming the TdT enzyme solution, centrifuge the tube for 30 seconds to force all the liquid to the bottom of the tube.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

pictograms

FlameHealth hazard

signalword

Danger

Hazard Classifications

Carc. 1B - Eye Irrit. 2 - Flam. Liq. 2 - Resp. Sens. 1

Storage Class

3 - Flammable liquids

wgk_germany

WGK 3

flash_point_f

69.8 °F

flash_point_c

21 °C


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