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11721925001

Roche

DNA Molecular Weight Marker XIII (50 bp ladder)

pkg of 50 μg (250 μg/ml), solution

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Synonym(s):
DNA marker

form

solution

Quality Level

packaging

pkg of 50 μg (250 μg/ml)

manufacturer/tradename

Roche

concentration

250 μg/mL

color

colorless

solubility

water: miscible

storage temp.

−20°C

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This Item
P95771172193300111062590001
vibrant-m

P9577

PCR 50 - 2,000 bp Marker

Quality Level

100

Quality Level

200

Quality Level

100

Quality Level

100

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

color

colorless

color

-

color

colorless

color

colorless

concentration

250 μg/mL

concentration

-

concentration

250 μg/mL

concentration

250 μg/mL

solubility

water: miscible

solubility

-

solubility

water: miscible

solubility

water: miscible

General description

Fragment mixture prepared by cleavage of a specially constructed plasmid with restriction endonucleases.
Size Range: 50 to 750 bp and an additional band of 2642 bp

Application

DNA Molecular Weight Marker XIII (50 bp ladder) allows accurate sizing of DNA fragments generated by PCR or restriction digest, followed by separation on agarose gels. DNA Molecular Weight Marker XIII (50 bp ladder) can be used in conjunction with DNA Molecular Weight Marker XIV (100 bp ladder) for precise size determination.
The fragments have 5′-protruding ends and can be labeled with radioactive nucleotides (e.g.,32P-dTTP or 32P-dGTP) by standard filling-in reactions. End-labeling reactions can be performed with a radioactive or nonradioactive dideoxynucleotide (e.g., DIG-11-ddUTP) and Terminal Transferase.
DNA Molecular Weight Marker for agarose gels; simplifies accurate molecular weight determination of double-stranded DNA fragments generated by:
  • Restriction digests
  • PCR
  • RT-PCR

Sequence

The mixture contains 15 double-stranded DNA fragments with the following base pair lengths (1 base pair = 660 daltons): 50, 100, 150, 200, 250, 300, 350, 400, 450, 500, 550, 600, 650, 700, 750, and an additional band of 2642 bp.
Note
: Fragment lengths are derived from computer analysis of the DNA sequence. Depending on the size range of the marker, the smallest fragments will only be visible on overloaded gels. The 250- and 500-bp banding pattern is two to three times brighter.

Unit Definition

50 μg = 1A260 unit

Physical form

Ready-to-use solution, 250μg/ml, in TE buffer (10mM Tris-HCl, 1mM EDTA, pH 8.0).

Preparation Note

Storage conditions (working solution): 2 to 8 °C
Once thawed we recommend further storage at 2 to 8 °C.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Storage Class

12 - Non Combustible Liquids

wgk_germany

nwg

flash_point_f

does not flash

flash_point_c

does not flash


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