MilliporeSigma
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11746081001

Roche

Nitrite/Nitrate, Colorimetric Test

sufficient for ~64 assays, pkg of 1 tests (5 components), suitable for water monitoring

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Synonym(s):
Nitrate, Nitrite, colormetric test

usage

sufficient for ~64 assays

packaging

pkg of 1 tests (5 components)

manufacturer/tradename

Roche

application(s)

water monitoring

storage temp.

2-8°C

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packaging

pkg of 1 tests (5 components)

packaging

-

packaging

-

packaging

-

manufacturer/tradename

Roche

manufacturer/tradename

-

manufacturer/tradename

-

manufacturer/tradename

-

application(s)

water monitoring

application(s)

-

application(s)

-

application(s)

-

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

General description

Linearity
The method is linear in the range from 0.02 mg nitrite or nitrate/l sample solution to 5.00 mg nitrite or nitrate/l sample solution.

Detection limit: 0.02mg/l for nitrite and nitrate

Specificity

Under the provided conditions, nitrate reductase reacts specifically with nitrate ions.

Application

Semimicro method for the determination of nitrite and/or nitrate to investigate water, waste water, environmental samples, plant material, foodstuffs, drugs, cosmetics, and biological samples. Nitrite/Nitrate, Colorimetric Test was used in a study done to estimate the in vivo effects of myrtle oil (Myrtii oleum) on the antioxidant enzymes such as catalase and superoxide dismutase in alloxan and normoglycaemic diabetic rabbits.

Packaging

1 test combination containing 5 components.

Preparation Note

Working solution: Preparation of the solutions
  • Use the contents of bottle #1 undiluted, bring to 15 to 25 °C before use.
  • Place one Coenzyme-tablet in a beaker and dissolve in 3 ml of the solution from bottle 1. Remove the Coenzyme-tablet using the tweezers provided. The resulting solution is reaction mixture 2 and is sufficient for 12 nitrate determinations, bring to 15 to 25 °C before use.
  • Dissolve the contents of one bottle #3 in 0.7 ml of double-dist. water to give solution 3.
  • Use the contents of one bottle #4 undiluted.
  • Use the contents of one bottle #5 undiluted.

Storage conditions (working solution):
  • Prepare Reaction Mixture 2 immediately before use.
  • Solution of Nitrate reductase is stable at 2 to 8 °C for 2 weeks.
  • Solution of Color reagent I is stable at 2 to 8 °C for 3 months.
  • Solution of Color reagent II is stable at 2 to 8 °C for 3 months.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Kit Components Only

Product No.
Description

  • Potassium phosphate buffer, stabilized, pH 7.5

  • NADPH, tablets, each containing NADPH and stabilizers

  • Nitrate reductase, lyophilized, 8 U

  • Color Reagent I, consisting of sulfanilamide and stabilizers

  • Color Reagent II, consisting of N-(1-naphthyl)-ethylenediamine dihydrochloride and stabilizers

Pictograms

CorrosionHealth hazard

Signal Word

Danger

Hazard Statements

Hazard Classifications

Eye Dam. 1 - Met. Corr. 1 - Resp. Sens. 1 - Skin Sens. 1

Storage Class Code

8B - Non-combustible, corrosive hazardous materials

WGK

WGK 2

Flash Point(F)

does not flash

Flash Point(C)

does not flash


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