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G4410

Sigma-Aldrich

Griess reagent (modified)

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Synonym(s):
Greiss, nitrite assay
EC Number:
NACRES:
NA.32

form

powder

Quality Level

usage

10 g

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1.09023MAK36738497
Griess reagent (modified)

G4410

Griess reagent (modified)

Nitrite Assay Kit (Griess Reagent) sufficient for 200 colorimetric tests

MAK367

Nitrite Assay Kit (Griess Reagent)

Millipore

38497

Nitrate Reagent A

form

powder

form

liquid

form

-

form

-

usage

10 g

usage

-

usage

sufficient for 200 colorimetric tests

usage

-

General description

Griess reagent is based on Griess reaction. The components include naphthylethylenediamine dihydrochloride suspended in water and sulphanilamide in phosphoric acid. This reagent reacts with nitrite in samples to form a purple azo product, absorbance of which is measured at 546 nm.

Application

Griess reagent (modified) has been used:
  • in the nitrite assay of primary spinal cord glial cells stimulated with supernatant of damaged sensory neuron (SDSN)
  • for measuring nitrite/nitrate oxidized from nitric oxide in stimulated chondrocytes
  • for measuring nitric oxide (NO) degradation in RAW264.7 macrophage cells
  • to measure the concentration of nitric oxide(NO) in the conditioned media from cultured macrophages (MΦs)
  • to determine the relative levels of NO released by BEAS-2B bronchial cells in the culture supernatants

pictograms

Corrosion

signalword

Danger

hcodes

Hazard Classifications

Eye Dam. 1 - Skin Corr. 1

Storage Class

8A - Combustible, corrosive hazardous materials

wgk_germany

WGK 2


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