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AGRLE-RO

Roche

Agarose LE

low electroendosmosis

Synonym(s):
agarose

form

powder

packaging

pkg of 100 g (11685660001)
pkg of 500 g (11685678001)

manufacturer/tradename

Roche

shipped in

ambient

storage temp.

20-25°C

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11696505001GE17-0554-02PPB013
Agarose LE low electroendosmosis

Roche

AGRLE-RO

Agarose LE

Agarose NA Cytiva 17-0554-02, pack of 100 g

GE17-0554-02

Agarose NA

packaging

pkg of 100 g (11685660001)

packaging

kit of for up to 100 reactions

packaging

pack of 100 g

packaging

-

manufacturer/tradename

Roche

manufacturer/tradename

Roche

manufacturer/tradename

Cytiva 17-0554-02

manufacturer/tradename

-

shipped in

ambient

shipped in

-

shipped in

-

shipped in

-

storage temp.

20-25°C

storage temp.

-

storage temp.

-

storage temp.

-

General description

Agarose is obtained from seaweed and contains repeated agarobiose units. During agarose gel formation, the polymers aggregate to form a network with varying pore sizes. This property is used in agarose gel electrophoresis to separate DNA.

Application

Agarose LE has been used for:

  • analyzing fragments between 0.2 and 15 kb
  • analysis of PCR products
  • examination of restriction endonucleases
  • digests of plasmid, cosmid, and λ phage DNA
  • electrophoresis of RNA in, e.g., denaturing gels containing formaldehyde
  • in combination with ethidium bromide ​in DNA electrophoresis to facilitate the separation and visualization of DNA fragments.
Nucleic acid fragments separated with Agarose LE can be blotted to nylon or nitro-cellulose membranes by all standard blotting techniques.
Important Note: Detection with nonradioactive probes e.g., digoxigenin-labeled nucleic acids, does not interfere with the use of Agarose LE.

Quality

Absence of DNase: none detected according to the current Quality Control procedures.
Absence of RNase: none detected according to the current Quality Control procedures.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

does not flash

Flash Point(C)

does not flash


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Agarose NA Cytiva 17-0554-02, pack of 100 g

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"Impact of Interleukin 16 (IL-16) Gene Polymorphism among

Seropositive Stages in HIV-1 Infected Patients in North India.
Kakkar K
Journal of Virology & Antiviral Research, 8, 006-011 (2016)
Pei Yun Lee et al.
Journal of visualized experiments : JoVE, 62(62), 3923-3923 (2012-05-02)
Agarose gel electrophoresis is the most effective way of separating DNA fragments of varying sizes ranging from 100 bp to 25 kb(1). Agarose is isolated from the seaweed genera Gelidium and Gracilaria, and consists of repeated agarobiose (L- and D-galactose)

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