All Photos(2)




Expand High FidelityPLUS PCR System

sufficient for ≤500 reactions (03300226001), sufficient for ≤2,500 reactions (03300234001), suitable for PCR, High Fidelity PCR, hotstart: no, dNTPs included: no

Sign Into View Organizational & Contract Pricing

high fidelity pcr plus system, expand
Enzyme Commission number:


sufficient for ≤2,500 reactions (03300234001)
sufficient for 2500 reactions
sufficient for ≤500 reactions (03300226001)
sufficient for 500 reactions

Quality Level


High Fidelity PCR
dNTPs included: no
hotstart: no


pkg of 2,500 U (03300234001 [10x250 U])
pkg of 500 U (03300226001 [2x250 U])




72 °C optimum reaction temp.


PCR: suitable


purified DNA

storage temp.


General description

The Expand High FidelityPLUS PCR System is a blend of Taq DNA polymerase and a thermostable proofreading protein that lacks polymerase activity.
The Expand High FidelityPLUS PCR System is designed to amplify fragments up to 5 kb from all types of DNA with high yield and fidelity. It amplifies DNA with sixfold greater fidelity than Taq DNA polymerase alone.
Expand High FidelityPLUS PCR System also incorporates dUTP. Cross-contamination can therefore be prevented by a pretreatment of dUTP containing DNA with heat-labile Uracil-DNA Glycosylase before amplification. It is suited for the simultaneous amplification of a large number of targets.


The Expand High FidelityPLUS PCR System designed for the robust amplification of fragments up to 5 kb from all types of DNA with outstanding yield, specificity, sensitivity, and accuracy. This kit is the product of choice for the development of a new amplification assay or the optimization of an existing assay.
Use Expand High FidelityPLUS PCR System for:
  • PCR
  • RT-PCR
  • Primer extension
  • Carryover prevention

Features and Benefits

  • Safeguard against cross-contamination.
This is the choice for large numbers of amplifications.
  • Obtain sixfold higher fidelity and higher yields,
compared to Taq DNA Polymerase.
  • Efficiently label DNA.
Use this kit to label DNA with radioactive or nonradioactive nucleotides.


1 kit containing 4 components


Each lot is PCR function-tested using human genomic DNA and EPO primers specific for a 1.8kb PCR product, and tPA primers specific for a 4.8kb PCR product.

Unit Definition

Volume Activity: 5 U/μL

Other Notes

For life science research only. Not for use in diagnostic procedures.

Legal Information

Transition-metal-catalyzed cross-coupling is an efficient tool for constructing C-C, C-N and C-O bonds in organic synthesis. Intensive interest has been focused on the development of the most effective ligands that improve catalyst performance. Ligands play a key role in stabilizing and activating the central metal atom and fine-tuning the selectivity of transformation. Phosphines have proven to be the most significant class of ligands for cross couplings, such as Negishi, Suzuki, Stille, Heck, Sonogashira, and Buchwald-Hartwig cross-coupling.
Expand is a trademark of Roche

Kit Components Only

Product No.

  • Expand High FidelityPLUS Enzyme Blend 5 U/μl

  • Expand High FidelityPLUS Reaction Buffer, with 7.5 mM MgCl2 5x concentrated

  • Expand High FidelityPLUS Reaction Buffer, without MgCl2 5x concentrated

  • MgCl2 Stock Solution 25 mM


Hazard Classifications

Aquatic Chronic 3

Storage Class

12 - Non Combustible Liquids




does not flash


does not flash

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).


Product Number
Pack Size/Quantity

Additional examples:





enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service