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EHIFIP-RO

Roche

Expand High FidelityPLUS PCR System

sufficient for ≤500 reactions (03300226001), sufficient for ≤2,500 reactions (03300234001), suitable for PCR, High Fidelity PCR, hotstart: no, dNTPs included: no

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Synonym(s):
high fidelity pcr plus system, expand
Enzyme Commission number:

usage

sufficient for ≤2,500 reactions (03300234001)
sufficient for 2500 reactions
sufficient for ≤500 reactions (03300226001)
sufficient for 500 reactions

Quality Level

feature

High Fidelity PCR
dNTPs included: no
hotstart: no

packaging

pkg of 2,500 U (03300234001 [10x250 U])
pkg of 500 U (03300226001 [2x250 U])

manufacturer/tradename

Roche

parameter

72 °C optimum reaction temp.

technique(s)

PCR: suitable

input

purified DNA

storage temp.

−20°C

General description

The Expand High FidelityPLUS PCR System is a blend of Taq DNA polymerase and a thermostable proofreading protein that lacks polymerase activity.
The Expand High FidelityPLUS PCR System is designed to amplify fragments up to 5 kb from all types of DNA with high yield and fidelity. It amplifies DNA with sixfold greater fidelity than Taq DNA polymerase alone.
Expand High FidelityPLUS PCR System also incorporates dUTP. Cross-contamination can therefore be prevented by a pretreatment of dUTP containing DNA with heat-labile Uracil-DNA Glycosylase before amplification. It is suited for the simultaneous amplification of a large number of targets.

Application

The Expand High FidelityPLUS PCR System designed for the robust amplification of fragments up to 5 kb from all types of DNA with outstanding yield, specificity, sensitivity, and accuracy. This kit is the product of choice for the development of a new amplification assay or the optimization of an existing assay.
Use Expand High FidelityPLUS PCR System for:
  • PCR
  • RT-PCR
  • Primer extension
  • Carryover prevention

Features and Benefits

  • Safeguard against cross-contamination.
This is the choice for large numbers of amplifications.
  • Obtain sixfold higher fidelity and higher yields,
compared to Taq DNA Polymerase.
  • Efficiently label DNA.
Use this kit to label DNA with radioactive or nonradioactive nucleotides.

Packaging

1 kit containing 4 components

Quality

Each lot is PCR function-tested using human genomic DNA and EPO primers specific for a 1.8kb PCR product, and tPA primers specific for a 4.8kb PCR product.

Unit Definition

Volume Activity: 5 U/μL

Other Notes

For life science research only. Not for use in diagnostic procedures.

Legal Information

Transition-metal-catalyzed cross-coupling is an efficient tool for constructing C-C, C-N and C-O bonds in organic synthesis. Intensive interest has been focused on the development of the most effective ligands that improve catalyst performance. Ligands play a key role in stabilizing and activating the central metal atom and fine-tuning the selectivity of transformation. Phosphines have proven to be the most significant class of ligands for cross couplings, such as Negishi, Suzuki, Stille, Heck, Sonogashira, and Buchwald-Hartwig cross-coupling.
Expand is a trademark of Roche

Kit Components Only

Product No.
Description

  • Expand High FidelityPLUS Enzyme Blend 5 U/μl

  • Expand High FidelityPLUS Reaction Buffer, with 7.5 mM MgCl2 5x concentrated

  • Expand High FidelityPLUS Reaction Buffer, without MgCl2 5x concentrated

  • MgCl2 Stock Solution 25 mM

hcodes

Hazard Classifications

Aquatic Chronic 3

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

does not flash

flash_point_c

does not flash


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