MilliporeSigma
All Photos(3)

Documents

FHIFIN-RO

Roche

FastStart High Fidelity PCR System, dNTPack

Sign Into View Organizational & Contract Pricing

Synonym(s):
PCR Kit, dntp

usage

sufficient for ≤200 reactions (04738292001)
sufficient for ≤50 reactions (04738284001)

Quality Level

packaging

pkg of 125 U (04738284001)
pkg of 500 U (04738292001 [2 x 250 U])

manufacturer/tradename

Roche

parameter

72 °C optimum reaction temp.

technique(s)

PCR: suitable

storage temp.

−20°C

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
EHIFIP-ROEHIFIDNTP-RO12140314001
vibrant-m

12140314001

High Fidelity PCR Master

usage

sufficient for ≤200 reactions (04738292001)

usage

sufficient for ≤2,500 reactions (03300234001), sufficient for 2500 reactions, sufficient for ≤500 reactions (03300226001), sufficient for 500 reactions

usage

sufficient for ≤1,000 reactions (04738276001), sufficient for ≤200 reactions (04738268001), sufficient for ≤40 reactions (04738250001)

usage

sufficient for ≤200 reactions

packaging

pkg of 125 U (04738284001)

packaging

pkg of 2,500 U (03300234001 [10x250 U]), pkg of 500 U (03300226001 [2x250 U])

packaging

pkg of 2,500 U (04738276001 [10x250 U]), pkg of 100 U (04738250001), pkg of 500 U (04738268001 [2x250 U])

packaging

kit of 1 (2 components)

manufacturer/tradename

Roche

manufacturer/tradename

Roche

manufacturer/tradename

Roche

manufacturer/tradename

Roche

parameter

72 °C optimum reaction temp.

parameter

72 °C optimum reaction temp.

parameter

-

parameter

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

General description

The enzyme dNTPack comprises the FastStart High Fidelity PCR System and a ready-to-use solution of PCR grade nucleotides. The FastStart High Fidelity PCR System is a blend of FastStart Taq DNA Polymerase and a thermostable proofreading protein, which is also chemically modified. This protein mediates proofreading activity, but carries no polymerase activity. Both proteins are inactive below +75°C and are activated by heating to +95°C for two minutes. The FastStart® High Fidelity PCR System is the ideal tool for all amplification experiments where a combination of high specificity, sensitivity, accuracy, and yield is needed. Hot start protocols have improved specificity, sensitivity, and PCR yield. Heat-labile blocking groups on amino acid residues of FastStart Taq DNA Polymerase make the enzyme inactive at room temperature. There is no elongation at low temperatures when primers bind nonspecifically.

Application

The FastStart® High Fidelity PCR System – an optimized enzyme blend for hot start PCR – combines all the benefits of FastStart Taq DNA Polymerase with fourfold higher accuracy and the ability to amplify fragments up to 5kb.
  • Hot start PCR
  • Multiplex PCR
  • Hot Start RT-PCR
  • GC-rich template amplification
  • Difficult or challenging PCRs
  • precapture ligation-mediated PCR (LM-PCR)
  • PCR amplification of cDNA 16S rRNA gene
The FastStart High Fidelity PCR System is the product of choice for multiplex PCR

Features and Benefits

  • Achieve excellent multiplex performance: For fast high quality results, also use Roche′s PCR Optimization Kit.
  • Increase fidelity: Achieve fourfold higher fidelity compared to Taq DNA Polymerase and FastStart Taq DNA Polymerase.
  • Amplify challenging DNA: DMSO, a PCR additive, for working with difficult templates, is supplied.
  • Cost-effective: Use the convenient premixed solution of PCR grade dNTPs.

Packaging

1 kit containing 6 components
  • FastStart High Fidelity Enzyme Blend, (5 U/μl) in storage buffer
  • FastStart High Fidelity Reaction Buffer, 10x concentrated, with 18mM MgCl2
  • FastStart High Fidelity Reaction Buffer, 10x concentrated, without MgCl2
  • MgCl2 Stock Solution, 25mM
  • DMSO
  • PCR Nucleotide Mix (each dNTPack contains the 10mM additive-free sodium salt nucleotides in a ready-to-use mix)

Quality

Each lot is PCR function-tested using human genomic DNA and primers specific for a 1.8 kb fragment of the EPO gene, and primers specific for a 4.8 kb fragment of the tPA gene.

Unit Definition

Volume Activity: 5 U/μl

Preparation Note

Working concentration: For a standard 50 μl PCR, we recommend using 2.5 U of the enzyme blend.

Other Notes

Contents
  • FastStart High Fidelity Enzyme Blend, (5 U/μl) in storage buffer
  • FastStart High Fidelity Reaction Buffer, 10x concentrated, with 18 mM MgCl2
  • FastStart High Fidelity Reaction Buffer, 10x concentrated, without MgCl2
  • MgCl2 Stock Solution, 25 mM
  • DMSO
  • PCR Nucleotide Mix
Each dNTPack contains the 10 mM additive-free sodium salt nucleotides in a ready-to-use mix.

Legal Information

NOTICE TO PURCHASER: LIMITED LICENSE<br />Use of this product is covered by US Patent No. 6,127,155 and corresponding patent claims outside the US.  The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research.  No right under any other patent claim and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel.  This product is for research use only. Human and veterinary diagnostic uses under Roche patent claims require a separate license from Roche.  All uses other than internal research and human and veterinary diagnostic uses under Roche patent claims require a separate license from Thermo Fisher Scientific. Further information on purchasing licenses from Roche may be obtained by contacting the Licensing Department of Roche Molecular Systems, Inc., 4300 Hacienda Drive, Pleasanton, California 94588, USA or Roche Diagnostics GmbH, Sandhofer Strasse 116, 68305 Mannheim, Germany.  Further information on purchasing licenses from Thermo Fisher Scientific may be obtained by contacting the Licensing Department of  Thermo Fisher Scientific, 5791 Van Allen Way, Carlsbad, California 92008, USA.</ br>NOTICE TO PURCHASER: LIMITED LICENSE<br />Use of this product is covered by US Patent Nos. 5,677,152 and 5,773,258, and corresponding patent claims outside the US.  The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research.  No right under any other patent claim and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel.  This product is for research use only.  Human and veterinary diagnostic uses under Roche patent claims require a separate license from Roche.  All uses other than internal research and human and veterinary diagnostic uses under Roche patent claims require a separate license from Thermo Fisher Scientific. Further information on purchasing licenses from Roche may be obtained by contacting the Licensing Department of Roche Molecular Systems, Inc., 4300 Hacienda Drive, Pleasanton, California 94588, USA or Roche Diagnostics GmbH, Sandhofer Strasse 116, 68305 Mannheim, Germany.  Further information on purchasing licenses from Thermo Fisher Scientific may be obtained by contacting the Licensing Department of  Thermo Fisher Scientific, 5791 Van Allen Way, Carlsbad, California 92008, USA.
FastStart is a trademark of Roche
Roche is a registered trademark of Roche Molecular Systems, Inc.

Kit Components Only

Product No.
Description

  • FastStart High Fidelity Enzyme Blend, in storage buffer 5 U/μl

  • FastStart High Fidelity Reaction Buffer, with 18 mM MgCl2 10x concentrated

  • FastStart High Fidelity Reaction Buffer, without MgCl2 10x concentrated

  • MgCl2 Stock Solution 25 mM

  • DMSO

  • PCR Nucleotide Mix

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

203.0 °F

flash_point_c

95 °C


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Customers Also Viewed

Slide 1 of 4

1 of 4

GC-RICH PCR System sufficient for &#8804;50&#160;reactions, pkg of 100&#160;U, optimum reaction temp. 72 °C

Roche

12140306001

GC-RICH PCR System

Pwo Master sufficient for &#8804;100&#160;reactions, pkg of 2.5&#160;mL (10 x 250 &#956;l)

Roche

03789403001

Pwo Master

Microbiota alter metabolism and mediate neurodevelopmental toxicity of 17beta-estradiol
Scientific reports, 9, 7064-7064 (2019)
Cornelia Adlhoch et al.
Veterinary microbiology, 139(3-4), 270-278 (2009-07-15)
Swine Hepatitis E virus (HEV) can be transmitted from pigs to humans causing hepatitis. A high prevalence of HEV in wild boar populations is reported for several European countries, but actual data for Germany are missing. During the hunting season
Hongmei Jing et al.
PloS one, 8(11), e79423-e79423 (2013-11-16)
Community composition of Bacteria in the surface and deep water layers were examined at three oceanic sites in the Pacific Ocean separated by great distance, i.e., the South China Sea (SCS) in the western tropical Pacific, the Costa Rica Dome
Differential contribution of cis and trans gene transcription regulatory mechanisms in amygdala and prefrontal cortex and modulation by social stress
Reuveni E, et al
Scientific reports, 8, 6339-6339 (2018)
Matthew J Neave et al.
Microbial ecology, 63(3), 639-650 (2011-11-01)
Tolerant species of polychaete worms can survive in polluted environments using various resistance mechanisms. One aspect of resistance not often studied in polychaetes is their association with symbiotic bacteria, some of which have resistance to metals and may help the

Articles

The purpose of Hot Start PCR is to inhibit the PCR reaction in order to reduce nonspecific amplification, prevent the formation of primer dimers, and increase product yields.

The possible causes and potential remedies for challenges encountered during preparation of samples for SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis) and optimizing electrophoresis conditions.

Related Content

Source a comprehensive range of quality reagents, reference materials and accessories for clinical and forensic testing across a range of testing methodologies including LC-MS, Molecular diagnostics and immunoassays. Bulk and customization options are available for enhancing efficiencies.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service