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0029-BP

Sigma-Aldrich

GlcNAcβ-C3-PAA-biot

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UNSPSC Code:
12352201
NACRES:
NA.25

carrier

PAA carrier

conjugate

biotin conjugate

product line

BP-Series

assay

95% (H-NMR)

form

lyophilized powder

mol wt

~20,000 Da

manufacturer/tradename

GlycoNZ

extent of labeling

20 mol% Biotin ligand density

technique(s)

flow cytometry: suitable
glycol-bead construction: suitable
glycol-surface construction: suitable

probe type

monosaccharide

probe presentation

polymeric

application(s)

histochemistry

storage temp.

−20°C

Related Categories

General description

BP-Series: Biotinylated Polymeric Probes
This series of glycoconjugate probes includes HOCH2CH2NH3+-salts for acidic sugars. The affinity of these probes is 102 to 105 times higher than that of the corresponding free sugars. The spacer-arm is normally –(CH2)3–, while the spacer arm for biotin is –(CH2)6–. The flexible polymer chain behaves as an additional spacer.

Used as a high molecular weight carrier, poly[N-(2-hydroxyethyl)acrylamide], abbreviated as PAA, has low non-specific sorption, and is stable to chemical and proteolytic action.

Application

Glycoconjugates are used to study the functions of carbohydrates, carbohydrate-binding proteins in biological processes, and on the discovery of new carbohydrate-mediated interactions.

Preparation Note

Purity: synthetic ω-aminoalkyl glycosides used for coupling with the polymer have a purity of >95% (HPLC and 1H-NMR)

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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