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08069

SAFC

modified Tryptic Soy Broth- Dehydrated Culture Media

NutriSelect® Plus, with bile salts mixture, according to ISO 16654:2001, powder, selective for enterovirulent E. coli (EEC) O157:H7

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Synonym(s):
Modified Soyabean Bile Broth, Modified soybean bile broth base, Modified tryptone soy broth, Tryptic soy broth, modified, mTSB
NACRES:
NA.85

agency

according to ISO 16654:2001

Quality Level

sterility

non-sterile

form

powder

shelf life

limited shelf life, expiry date on the label

composition

casein enzymic hydrolysate, 17 g/L
dipotassium hydrogen phosphate, 4 g/L
D-glucose, 2.5 g/L
papaic digest of soyabean meal, 3 g/L
sodium chloride, 5 g/L
with bile salts mixture

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbiological culture: suitable

final pH

7.3±0.2 (25 °C)

application(s)

food and beverages
microbiology
pathogen testing
vitamins, nutraceuticals, and natural products

suitability

selective for enterovirulent E. coli (EEC) O157:H7

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This Item
1.40011B311-200DSV311-15GS
form

powder

form

granular

form

liquid

form

liquid

sterility

non-sterile

sterility

-

sterility

sterile; autoclaved

sterility

-

suitability

selective for enterovirulent E. coli (EEC) O157:H7

suitability

Escherichia coli O157:H7, Escherichia coli (non-O157 Shiga Toxin-Producing (STEC)), Salmonella spp.

suitability

nonselective for

suitability

nonselective for

application(s)

food and beverages
microbiology
pathogen testing
vitamins, nutraceuticals, and natural products

application(s)

food and beverages, microbiology
pathogen testing

application(s)

food and beverages
pharmaceutical

application(s)

food and beverages
pharmaceutical

shelf life

limited shelf life, expiry date on the label

shelf life

-

shelf life

-

shelf life

-

Application

Modified tryptone soy broth is recommended as an enrichment medium for the detection of Escherichia coli O157:H7 from food and animal feeding stuffs.

Preparation Note

Suspend 33 grams of Modified tryptone soy broth in 1000 ml distilled water. Heat if necessary to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121 °C) for 15 minutes. Cool to 45-50 °C and aseptically add rehydrated contents of 1 vial of VCC Selective Supplement (Cat. No. No. 80704). Mix well and dispense as desired.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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