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T8907

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Tryptic Soy Broth - Dehydrated Culture Media

NutriSelect® Basic, powder, suitable for microbiology

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Synonym(s):
Soybean Casein digest Broth, Casein Soya Broth, CASO Broth, TSB, CASO Broth, Casein Soya Broth, Soybean Casein digest Broth, TSB, Tryptone Soya Broth
MDL number:
NACRES:
NA.85

sterility

non-sterile

Quality Level

form

powder

quality

Microbiologically tested.

packaging

poly bottle of 1 kg
poly bottle of 500 g

manufacturer/tradename

NutriSelect® Basic

application(s)

environmental
food and beverages
pathogen testing
pharmaceutical

microbiology

suitability

bacteria

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This Item
22092MHA000T2B1.46317
Tryptic Soy Broth pkg of 2 mL, ampoule non-selective medium used to detect total heterotrophic microorganisms in water and other liquids

Millipore

MHA000T2B

Tryptic Soy Broth

Tryptic Soy Broth bottle of, ready-to-use, bottle volume 100 mL , filling volume

Millipore

1.46317

Tryptic Soy Broth

form

powder

form

powder

form

liquid

form

liquid (ready-to-use)

packaging

poly bottle of 1 kg, poly bottle of 500 g

packaging

bottle of 500 g

packaging

pkg of 2 mL, pack of 50 tests

packaging

bottle of

manufacturer/tradename

NutriSelect® Basic

manufacturer/tradename

NutriSelect® Plus

manufacturer/tradename

-

manufacturer/tradename

-

application(s)

environmental
food and beverages
pathogen testing
pharmaceutical, microbiology

application(s)

bioburden testing
cleaning products
clinical testing
cosmetics
environmental
food and beverages
pathogen testing
personal care
pharmaceutical
sample preparation
veterinary
water monitoring, microbiology

application(s)

clinical
cosmetics
environmental
food and beverages
industrial qc
microbiology

application(s)

cosmetics
food and beverages
pharmaceutical
sterility testing

suitability

bacteria

suitability

nonselective for Bacillus spp., nonselective for Bacteroides spp., nonselective for Campylobacter spp., nonselective for Candida spp., nonselective for Escherichia coli, nonselective for Neisseria spp., nonselective for Staphylococcus spp., nonselective for Streptococcus spp., nonselective for bacteria (General Media), nonselective for coliforms

suitability

nonselective for

suitability

nonselective for

General description

Tryptic Soy Broth is a general-purpose liquid medium used in qualitative procedures for the sterility testing, enrichment, and cultivation of fastidious microorganisms. The formulation contains a combination of casein and soy peptones supplying organic nitrogen, particularly amino acids and long-chained peptides. Sodium chloride maintains the osmotic equilibrium and dextrose serves as a source of carbohydrate and energy.

Application

Tryptic Soy Broth is a widely used medium for culturing aerobic gram-positive, gram-negative bacteria, fungus, routine blood cultures, and microbial biofilms. It is also been used as a growth medium to culture Staphylococcus aureus strains and as a growth medium for E.coli in the bacterial-killing assay (BKA) with bird plasma samples.

Components

Casein (pancreatic digest) 17 g/L
Soya peptone (papaic digest) 3 g/L
Sodium chloride 5 g/L
Dipotassium phosphate 2.5 g/L
Dextrose 2.5 g/L

Preparation Note

Suspend 30 g of dehydrated media in 1 litre of purified filtered water. Heat with frequent agitation and boil for one minute. Sterilize at 121°C for 15 minutes. Cool to 45-50°C. Mix gently and dispense into sterile Petri dishes or sterile culture tubes.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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