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Pseudomonas Isolation Agar

NutriSelect® Plus, suitable for microbiology, for primary isolation and identification of Pseudomonas

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Synonym(s):
Pseudomonas Agar
NACRES:
NA.85

Quality Level

sterility

non-sterile

form

powder

shelf life

limited shelf life, expiry date on the label

composition

agar, 13.6 g/L
magnesium chloride, 1.4 g/L
peptic digest of animal tissue, 20 g/L
potassium sulfate, 10 g/L
triclosan (Irgasan), 0.025 g/L

packaging

pkg of 500 g

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbiological culture: suitable

final pH

7.0±0.2 (25 °C)

application(s)

agriculture
clinical testing
environmental
food and beverages
water monitoring

microbiology

storage temp.

2-25°C

suitability

selective for Pseudomonas spp.

Related Categories

General description

Pseudomonas Isolation Agar is used as a selective and differential medium for primary isolation and identification of Pseudomonas aeruginosa. It is a modified medium based on the formulation of Medium A by King et al. and developed to differentiate Pseudomonas aeruginosa from other Pseudomonas sp. based on pigment formation. Peptic digest of animal tissue provides nitrogenous compounds. Glycerol serves as carbohydrate source and promotes the phycocyanin (blue-green pigment) production. Magnesium is a cofactor for many metabolic reactions and together potassium sulphate stimulates phyocyanin production as well. Irgasan is an antibiotic and selective inhibits gram-positive and gram-negative bacteria other than Pseudomonas spp. Agar is the solidifying agent.

Application

Pseudomonas Isolation Agar is used as a selective and differential medium for primary isolation and identification of Pseudomonas from clinical and nonclinical specimens.

Preparation Note

Suspend 45.03 g in 1 litre distilled water containing 20 ml glycerol (Cat. No. 49767). Boil to dissolve the medium completely. Sterilize by autoclaving at 121°C for 15 minutes.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves


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Asmaa T Y Kishawy et al.
Animals : an open access journal from MDPI, 10(10) (2020-09-27)
The aim of the present study was to evaluate mannan oligosaccharides (MOS) or glycerol (GLY) as a carbon source on biofloc systems of Nile tilapia (O. niloticus) juveniles. Fish (n = 750) were reared in open flow (Controls) or biofloc
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Climate change causes permafrost thawing, and we are confronted with the unpredictable risk of newly discovered permafrost microbes that have disease-causing capabilities. Here, we first characterized the detailed chemical structure of the lipid A moiety from a Pseudomonas species that

Articles

An article concerning the detection, identification, differentiation, and cultivation of Pseudomonas species.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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