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Millipore

GSP Agar

suitable for microbiology, NutriSelect® Plus

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Synonym(s):
Pseudomonas Aeromonas Selective Agar acc. to Kielwein
NACRES:
NA.74

sterility

non-sterile

Quality Level

form

powder

shelf life

limited shelf life, expiry date on the label

composition

agar, 12 g/L
magnesium sulfate, 0.5 g/L
phenol red, 0.36 g/L
potassium dihydrogen phosphate, 2 g/L
sodium L-glutamate, 10 g/L
starch (soluble), 20 g/L

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbiological culture: suitable

final pH

7.2±0.2 (25 °C)

application(s)

bioburden testing
environmental
food and beverages
water monitoring

microbiology

suitability

Aeromonas spp.
selective and differential for Pseudomonas spp.

Related Categories

General description

Agar is a complex gelatinous carbohydrate, and is added to the LB broth (Lysogeny broth, Luria broth, Lennox broth, or Luria-Bertani medium), in order to form gel for bacteria to grow upon. LB Broth is the most widely used nutritionally rich medium, for the culture and growth of bacteria. There are several different formulations of Lb broth, but the composition is generally the same comprising of peptides and casein peptones, trace elements, mineral and vitamins.

Application

For the detection of Pseudomonas and Aeromonas in all types of food as well as in the equipment and water supply of food-processing firms.

Preparation Note

Dissolve 45 g in 1 litre distilled water and sterilize by autoclaving at 121°C for 15 minutes. Cool to 45-50°C and add 100′000 IU/l Penicillin G (Cat. No. 13752; 60mg/l) and if desired 10 mg/l Pimaricin (Cat. No. 80482; 400ul/l). Mix well and pour into sterile petri plates.

Other Notes

A medium for selective cultivation of Pseudomonas and Aeromonas; The aerobic Pseudomonas - a taxonomic study

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


Certificates of Analysis (COA)

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R.Y. Stanier et al.
Journal of General Microbiology, 42, 159-159 (1966)
G. Kielwein
Archiv Fur Lebensmittelhygiene, 20, 131-131 (1969)
Archiv Fur Lebensmittelhygiene, 22, 15-15 (1971)
The structure of agar: Part I. Fractionation of a complex mixture of polysaccharides.
Duckworth M and Yaphe W.
Carbohydrate Research, 16 (1), 189-197 (1971)
Agar and agarose biotechnological applications.
Armisen R.
Hydrobiologia, 221 (1), 157-166 (1991)

Articles

An article concerning the detection, identification, differentiation, and cultivation of Pseudomonas species.

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