MilliporeSigma
All Photos(1)

Documents

70191

Millipore

Mueller Hinton Agar

suitable for microbiology, NutriSelect® Plus

Sign Into View Organizational & Contract Pricing

Synonym(s):
MH agar; Antibiotic sensitivity test media; disc diffusion test media
NACRES:
NA.74

sterility

non-sterile

Quality Level

product line

BioChemika

form

powder

shelf life

limited shelf life, expiry date on the label

composition

agar, 17.0 g/L
beef infusion solids, 2.0 g/L
casein hydrolysate, 17.5 g/L
starch, 1.5 g/L

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbe id | susceptibility testing: suitable
microbiological culture: suitable

final pH

7.3±0.2 (25 °C)

application(s)

clinical testing
food and beverages

microbiology

storage temp.

10-30°C

suitability

nonselective for Neisseria spp.
nonselective for bacteria (General Media)

Related Categories

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
975807019290922
Mueller Hinton Agar suitable for microbiology, NutriSelect® Plus

70191

Mueller Hinton Agar

Mueller-Hinton Agar 2 suitable for microbiology, NutriSelect® Plus

97580

Mueller-Hinton Agar 2

Mueller Hinton Broth suitable for microbiology, NutriSelect® Plus

70192

Mueller Hinton Broth

Mueller Hinton Broth 2 NutriSelect® Plus, Cation-adjusted, suitable for microbiology

90922

Mueller Hinton Broth 2

suitability

nonselective for Neisseria spp., nonselective for bacteria (General Media)

suitability

Haemophilus spp., nonselective for Enterococcus spp., nonselective for Escherichia coli, nonselective for Pseudomonas spp., nonselective for Staphylococcus spp., nonselective for Streptococcus spp., nonselective for bacteria (General Media), nonselective for coliforms

suitability

nonselective for bacteria (General Media)

suitability

nonselective for Enterococcus spp., nonselective for Escherichia coli, nonselective for Neisseria spp., nonselective for Pseudomonas spp., nonselective for Staphylococcus spp., nonselective for bacteria (General Media), nonselective for coliforms

technique(s)

microbe id | susceptibility testing: suitable, microbiological culture: suitable

technique(s)

microbe id | susceptibility testing: suitable, microbiological culture: suitable

technique(s)

microbe id | susceptibility testing: suitable, microbiological culture: suitable

technique(s)

microbe id | susceptibility testing: suitable, microbiological culture: suitable

sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

composition

agar, 17.0 g/L , casein hydrolysate, 17.5 g/L , beef infusion solids, 2.0 g/L , starch, 1.5 g/L

composition

agar, 17.0 g/L , beef heart infusion, 2 g/L , casein acid hydrolysate, 17.5 g/L , starch, soluble, 1.5 g/L

composition

beef infusion solids, 2.0 g/L , casein hydrolysate, 17.5 g/L , starch, 1.5 g/L

composition

acid hydrolysate of casein, 17.5 g/L , beef extract, 3 g/L , starch, 1.5 g/L

application(s)

clinical testing
food and beverages, microbiology

application(s)

clinical testing
food and beverages, microbiology

application(s)

clinical testing
food and beverages, microbiology

application(s)

clinical testing
food and beverages, microbiology

General description

Mueller Hinton Agar is specially designed for antibiotic susceptibility testing using the disc diffusion method (Kirby Bauer method). It has been recommended by the CLSI as the ideal medium for antibiotic susceptibility testing for the following reasons:

  • It shows good reproducibility from batch to batch
  • It is low in certain inhibitors like trimethoprim, sulfonamide and tetracycline
  • It supports the growth of both fastidious and non-fastidious bacterial pathogens
  • The starch in the media absorbs toxins thus preventing them from interfering with the antibiotics
  • It is a loose agar and therefore facilitates better diffusion of antibiotics
Mueller Hinton Agar has been used:
  • In culturing Escherichia coli ATCC™ 25922 strain for disc diffusion studies
  • In disk diffusion assay of fusidic acid against Staphylococcus aureus, Bacillus cereus, Pseudomonas aeruginosa and Escherichia coli
  • In agar diffusion assay of drug-loaded nano-hydroxyapatite formulations against Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli

application

Mueller Hinton Agar is a solid medium originally designed for the isolation of pathogenic Neisseria species, now widely used for antibiotic susceptibility testing (including sulfonamides) of aerobic and facultatively anaerobic bacteria isolated from clinical specimens.

Preparation Note

Suspend 38 g in 1 litre of distilled water, bring to the boil to dissolve the medium completely and sterilize by autoclaving at 121°C for 15 minutes.

Other Notes

Enhancement of cefotoxamine and other cephalosporins against Enterococcus faecalis by blood supplemented Mueller-Hinton agar

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Customers Also Viewed

Slide 1 of 4

1 of 4

Multi-drug delivery system based on lipid membrane mimetic coated nano-hydroxyapatite formulations
Placente D, et al.
International Journal of Pharmaceutics, 548(1), 559-570 (2018)
Mehmet Evren Okur et al.
Saudi pharmaceutical journal : SPJ : the official publication of the Saudi Pharmaceutical Society, 28(3), 338-348 (2020-03-21)
The objective of the present research was to examine the possible usage of microemulsion based gel for fusidic acid (FA) dermal application as burn wound treatment. During the preparation of microemulsion, ethyl oleate as oil phase, tween 80 as a
G M Eliopoulos et al.
Diagnostic microbiology and infectious disease, 12(2), 149-156 (1989-03-01)
The activities of cefotaxime and other aminothiazoyl oxime cephalosporins against Enterococcus faecalis were enhanced by addition of 5% sheep blood to Mueller-Hinton agar. This effect was not seen with aztreonam (aminothiazoyl oxime monobactam), cefotiam (aminothiazoyl, nonoxime), or other cephalosporins, and
Preparation and evaluation of QbD based fusidic acid loaded in situ gel formulations for burn wound treatment
Aksu NB, et al.
Journal of drug delivery science and technology, 52, 110-121 (2019)
Solid-state structure and antimicrobial and cytotoxicity studies of a cucurbit [6] uril-like Cu6L4 constructed from 3, 5-bis [(1H-tetrazol-5-yl) methyl]-4H-1, 2, 4-triazol-4-amine
Vasylevskyi S, et al.
Acta Crystallographica Section C, Structural Chemistry, 74(11) (2018)

Articles

Campylobacter spp. are regarded as the leading cause of bacterial gastroenteritis in humans worldwide.

Self-assembled monolayers (SAMs) have attracted enormous interest for a wide variety of applications in micro- and nano-technology. In this article, we compare the benefits of three different classes of SAM systems (alkylthiolates on gold).

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service