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229253

Sigma-Aldrich

Carminic acid

≥90% (HPLC)

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Synonym(s):
Natural Red 4
Empirical Formula (Hill Notation):
C22H20O13
CAS Number:
Molecular Weight:
492.39
Colour Index Number:
75470
Beilstein/REAXYS Number:
71651
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.47

Quality Level

assay

≥90% (HPLC)

form

powder

optical activity

[α]20/D +3.1°, c = 1 in H2O

color

purple to red-purple
red to very dark red

mp

136 °C

solubility

1 N acetic acid: 1%, clear to hazy, red

λmax

495 nm

ε (extinction coefficient)

≥13000 at 222-228  nm in ethanol at 0.03 g/L
≥18000 at 275-281 nm in ethanol at 0.03 g/L

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

Cc1c(C(O)=O)c(O)cc2C(=O)c3c(O)c(O)c([C@@H]4O[C@H](CO)[C@@H](O)[C@H](O)[C@H]4O)c(O)c3C(=O)c12

InChI

1S/C22H20O13/c1-4-8-5(2-6(24)9(4)22(33)34)13(25)10-11(15(8)27)16(28)12(18(30)17(10)29)21-20(32)19(31)14(26)7(3-23)35-21/h2,7,14,19-21,23-24,26,28-32H,3H2,1H3,(H,33,34)/t7-,14-,19+,20-,21+/m1/s1

InChI key

DGQLVPJVXFOQEV-JNVSTXMASA-N

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1 of 4

This Item
C1022C615211298
vibrant-m

229253

Carminic acid

vibrant-m

C1022

Carmine

vibrant-m

C6152

Carmine

vibrant-m

11298

Carminic acid

ε (extinction coefficient)

≥13000 at 222-228  nm in ethanol at 0.03 g/L, ≥18000 at 275-281 nm in ethanol at 0.03 g/L

ε (extinction coefficient)

-

ε (extinction coefficient)

-

ε (extinction coefficient)

-

assay

≥90% (HPLC)

assay

-

assay

-

assay

≥90% (HPLC)

λmax

495 nm

λmax

-

λmax

-

λmax

-

mp

136 °C

mp

-

mp

-

mp

-

solubility

1 N acetic acid: 1%, clear to hazy, red

solubility

ammonium hydroxide: 1 mg/mL

solubility

-

solubility

-

General description

Carmine is a cochineal product usually furnished as an aluminum and calcium salt of carminic acid. Carminic acid is used for human-medical cell diagnosis and serves the purpose of the histological investigation of sample material of human origin. It is suitable for staining nuclei in histological sections. It is utilized in various staining methods including the procedure for detection of glycogen and mucous substances (Best′s carmine), for nuclear staining, or vital staining.

Application

Carminic acid (CI 75470) is used in Orth′s lithium carmine technique for nuclear staining, Best′s stain for glycogen, and Southgates′s mucicarmine method.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 2

ppe

Eyeshields, Gloves, type N95 (US)


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