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O7380

Sigma-Aldrich

Orcein

synthetic

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Synonym(s):
Natural Red 28
CAS Number:
EC Number:
MDL number:
NACRES:
NA.47

biological source

synthetic

Quality Level

form

powder or crystals

solubility

1 M NaOH: 1 mg/mL, clear

εmax

≥400 at 580 nm in 0.1 M NaOH

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

InChI

1S/C28H24N2O7/c1-11-5-15(31)7-17(33)25(11)29-27-13(3)23-21(9-19(27)35)37-22-10-20(36)28(14(4)24(22)23)30-26-12(2)6-16(32)8-18(26)34/h5-10,29-34H,1-4H3

InChI key

VPEASJIRGSVXBF-UHFFFAOYSA-N

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This Item
O7505O0625S2255
Sigma-Aldrich

Sigma-Aldrich

O7380

Orcein

Orcein synthetic, certified by the Biological Stain Commission

Sigma-Aldrich

O7505

Orcein

Oil Red O certified by the Biological Stain Commission

Sigma-Aldrich

O0625

Oil Red O

Safranin O Dye content ≥85 %

Sigma-Aldrich

S2255

Safranin O

solubility

1 M NaOH: 1 mg/mL, clear

solubility

1 M NaOH: 1 mg/mL

solubility

chloroform/ethanol (1:1): 1 mg/mL

solubility

water: 1 mg/mL, clear, dark red to very dark red and red purple

εmax

≥400 at 580 nm in 0.1 M NaOH

εmax

≥250% at 574-582 nm in NaOH at 0.01 M

εmax

-

εmax

1250-1650 at 530-534 nm in 50% ethanol

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

Quality Level

100

Quality Level

-

Quality Level

200

Quality Level

200

General description

Orcein or CI Natural Red 28 is an elastic tissue stain. The fluorescence properties of Orcein show maximum emission peak at 587 nm (low concentration) and 620 nm (high concentration) upon suitable excitation and acidic pH.

Application

Orcein is highly recommended as a vital stain for elastic fibers and lamellae. Orcein is used as a nuclear stain for mounting embryos and assessing the maturation of oocytes. Other routine applications of orcein involve the staining of plant chromosomes.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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T1503
Product Number
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25G
Pack Size/Quantity

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705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

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Efficient decellularization of whole porcine kidneys improves reseeded cell behavior.
Poornejad N
Biomedical Materials (Bristol, England), 11, 025003-025003 (2016)
The effects of L-ergothioneine and L-ascorbic acid on the in vitro maturation (IVM) and embryonic development (IVC) of sheep oocytes.
OZTURKLER Y
Development, 5-5 (2010)
M L Molero et al.
Journal of microscopy, 144(Pt 1), 45-53 (1986-10-01)
The fluorescence properties of orcein are described in this paper. Under suitable exciting light, acidic solutions of orcein showed emission peaks at 587 nm (low concentration) and at 620 nm (high concentration). In fluorescence microscopy an intense orange-red emission was
Popescu P and Hayes H
Techniques in Animal Cytogenetics (2000)
Mariusz Gajda et al.
Histochemistry and cell biology, 147(6), 671-681 (2017-02-09)
Numerous cellular and extracellular components should be analyzed in sections of atherosclerotic plaques to assess atherosclerosis progression and vulnerability. Here, we combined orcein (O) staining for elastic fibers and martius scarlet blue (MSB) polychrome to visualize various morphological contents of

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