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S2255

Sigma-Aldrich

Safranin O

Dye content ≥85 %

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Synonym(s):
Basic Red 2, Cotton Red, Gossypimine, Safranin T, Safranin Y or A
Empirical Formula (Hill Notation):
C20H19ClN4
CAS Number:
Molecular Weight:
350.84
Colour Index Number:
50240
Beilstein/REAXYS Number:
3924099
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.47

form

powder

Quality Level

composition

Dye content, ≥85%

solubility

water: 1 mg/mL, clear, dark red to very dark red and red purple

εmax

1250-1650 at 530-534 nm in 50% ethanol

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Cl-].Cc1cc2nc3cc(C)c(N)cc3[n+](-c4ccccc4)c2cc1N

InChI

1S/C20H18N4.ClH/c1-12-8-17-19(10-15(12)21)24(14-6-4-3-5-7-14)20-11-16(22)13(2)9-18(20)23-17;/h3-11H,1-2H3,(H3,21,22);1H

Inchi Key

OARRHUQTFTUEOS-UHFFFAOYSA-N

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1 of 4

This Item
S88848412094635
vibrant-m

S2255

Safranin O

vibrant-m

S8884

Safranin O

vibrant-m

84120

Safranin O

vibrant-m

94635

Gram′s safranin solution

composition

Dye content, ≥85%

composition

Dye content, ≥80%

composition

-

composition

ethanol, 10%

εmax

1250-1650 at 530-534 nm in 50% ethanol

εmax

-

εmax

≥1000 at 525-535 nm in 50% ethanol

εmax

4.0 at 532 nm in 50% ethanol

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

form

powder

form

powder

form

powder or crystals

form

liquid

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology
microbiology

application(s)

diagnostic assay manufacturing
hematology
histology

General description

Safranin O is a cationic dye, which displays less significant metachromasia. It is used to detect any variations in articular disease. Safranin O binds specifically to polyanions. It detects the amount of proteoglycan present in cartilages. Safranin O is used as a marker for glycosaminoglycan depletion in osteoarthritis. Safranin is used as a counter stain in Gram staining.

application

Safranin O has been used in the histological analysis of femurs. It has also been used to detect the presence of glycosaminoglycans in the inner body of meniscus structures.

pictograms

Corrosion

signalword

Danger

hcodes

Hazard Classifications

Eye Dam. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

ppe

dust mask type N95 (US), Eyeshields, Gloves


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Delivery of PDGF-B and BMP-7 by mesoporous bioglass/silk fibrin scaffolds for the repair of osteoporotic defects
Yufeng Zhang
Biomaterials (2012)
Paul A. Banaszkiewicz
Classic Papers in Orthopaedics (2014)
A new modification and application of the Gram stain
Hucker GJ
Journal of Bacteriology, 6(4), 395-395 (1921)
Classic Papers in Orthopaedics (2014)
Maria D
Introduction to Diagnostic Microbiology for the Laboratory Sciences (2014)

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