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F7252

Sigma-Aldrich

Fast Green FCF

Dye content ≥85 %

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Synonym(s):
Food green 3
Linear Formula:
C37H34N2O10S3Na2
CAS Number:
Molecular Weight:
808.85
Colour Index Number:
42053
Beilstein:
5718212
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.83

form

powder

Quality Level

composition

Dye content, ≥85%

mp

290 °C (dec.) (lit.)

solubility

H2O: 1 mg/mL, clear

SMILES string

[Na+].[Na+].CCN(Cc1cccc(c1)S([O-])(=O)=O)c2ccc(cc2)C(=C3/C=CC(\C=C3)=[N+](\CC)Cc4cccc(c4)S([O-])(=O)=O)\c5ccc(O)cc5S([O-])(=O)=O

InChI

1S/C37H36N2O10S3.2Na/c1-3-38(24-26-7-5-9-33(21-26)50(41,42)43)30-15-11-28(12-16-30)37(35-20-19-32(40)23-36(35)52(47,48)49)29-13-17-31(18-14-29)39(4-2)25-27-8-6-10-34(22-27)51(44,45)46;;/h5-23H,3-4,24-25H2,1-2H3,(H3,41,42,43,44,45,46,47,48,49);;/q;2*+1/p-2

InChI key

RZSYLLSAWYUBPE-UHFFFAOYSA-L

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This Item
F7258L1886B4014
Fast Green FCF Dye content ≥85 %

Sigma-Aldrich

F7252

Fast Green FCF

Fast Green FCF certified by the Biological Stain Commission

Sigma-Aldrich

F7258

Fast Green FCF

Brilliant Green certified by the Biological Stain Commission

Sigma-Aldrich

B4014

Brilliant Green

solubility

H2O: 1 mg/mL, clear

solubility

H2O: 1 mg/mL

solubility

H2O: 1 mg/mL

solubility

H2O: 1 mg/mL, blue

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

composition

Dye content, ≥85%

composition

dye content, ≥90%

composition

Dye content, ≥65%

composition

Dye content, ≥90%

mp

290 °C (dec.) (lit.)

mp

290 °C (dec.) (lit.)

mp

288 °C (dec.) (lit.)

mp

210 °C (dec.) (lit.)

General description

Fast Green FCF is one of the triarylmethane dyes, that is biocompatible. This triphenylmethane color additive is mainly used in coloring food, drugs and cosmetics.

Application

Fast Green FCF has been used for in staining of menisci from rabbit and of osteochondral tissues.
Fast Green FCF dye is used for protein staining in IEF, native PAGE and SDS-PAGE. Fast Green staining is linear over a wider range of protein concentrations than Brilliant Blue R. After electrophoresis, fixing the proteins in the gel is recommended for maximum sensitivity. The gel can then be stained for 2 hr with 0.1% Fast Green FCF in either 30% (v/v) ethanol, 10% (v/v) acetic acid or in 7% acetic acid. The gel can be destained with either 30% (v/v) ethanol, 10% (v/v) acetic acid, or 7% acetic acid. The stained gel can be scanned at 625 nm. The detection sensitivity is approx. 30% that with Brilliant Blue R.

Biochem/physiol Actions

Fast Green FCF can be considered as a safe stain to detect tumor. In recent years, this has gained importance as an important drug to treat amyloid-deposit diseases.

Pictograms

Exclamation markHealth hazard

Signal Word

Warning

Hazard Statements

Hazard Classifications

Muta. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

352.4 °F - closed cup

Flash Point(C)

178 °C - closed cup

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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