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S8884

Sigma-Aldrich

Safranin O

certified by the Biological Stain Commission

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Synonym(s):
Basic Red 2, Cotton Red, Gossypimine, Safranin T, Safranin Y or A
Empirical Formula (Hill Notation):
C20H19ClN4
CAS Number:
Molecular Weight:
350.84
Colour Index Number:
50240
Beilstein/REAXYS Number:
3924099
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.47

grade

certified by the Biological Stain Commission

Quality Level

form

powder

composition

Dye content, ≥80%

color

green to very dark green

solubility

H2O: 1 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Cl-].Cc1cc2nc3cc(C)c(N)cc3[n+](-c4ccccc4)c2cc1N

InChI

1S/C20H18N4.ClH/c1-12-8-17-19(10-15(12)21)24(14-6-4-3-5-7-14)20-11-16(22)13(2)9-18(20)23-17;/h3-11H,1-2H3,(H3,21,22);1H

InChI key

OARRHUQTFTUEOS-UHFFFAOYSA-N

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1 of 4

This Item
S22558412094635
vibrant-m

S8884

Safranin O

vibrant-m

S2255

Safranin O

vibrant-m

84120

Safranin O

vibrant-m

94635

Gram′s safranin solution

color

green to very dark green

color

-

color

-

color

red to very dark red

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology
microbiology

application(s)

diagnostic assay manufacturing
hematology
histology

form

powder

form

powder

form

powder or crystals

form

liquid

solubility

H2O: 1 mg/mL

solubility

water: 1 mg/mL, clear, dark red to very dark red and red purple

solubility

methanol: 0.01 g/10 mL, red

solubility

water: soluble at 20 °C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

100

General description

Safranin O is a metachromatic, cationic dye. It is used as a counterstain in Gram staining. The stain colors Gram-negative bacteria pink to red and has no effect on Gram-positive bacteria. It is also used to assess glycosaminoglycans (GAGs) in cartilage. The dye imparts an intense red to GAGs. It easily binds to polyanions.

Application

Safranin O has been used in the staining of extraocular muscle mesenchymal stem cells (EOM-MSC) for the detection of cartilage, mucin and mast cell granules It has also been used as a mediator dye in cytochrome P450 spectral assay.
Safranin O has been used to stain matrix proteoglycans to study cartilaginous tissue structural integrity in mice. It has also been used to stain chondrogenically differentiated human bone marrow stromal cells.

Suitability

Certified as a stain for chromosomes in the Flemming triple stain; to stain gram negative bacteria in the Gram stain procedure; Safranine-fast green FCF stain for plant cells.

pictograms

Corrosion

signalword

Danger

hcodes

Hazard Classifications

Eye Dam. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

ppe

dust mask type N95 (US), Eyeshields, Gloves


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Classic Papers in Orthopaedics (2014)
Isolation of Multipotent Mesenchymal Stem Cells from Human Extraocular Muscle Tissue
Sharma A, et al.
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Perturbations in the HDL metabolic pathway predispose to the development of osteoarthritis in mice following long-term exposure to western-type diet
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Guengerich FP, et al.
Nature Protocols, 4(9), 1245-1245 (2009)

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