32211-M

Chloroform

puriss. p.a., reag. ISO, reag. Ph. Eur., 99.0-99.4% (GC)

• Synonym(s): Methylidyne trichloride, Trichloromethane
• Empirical Formula (Hill Notation): CHCl₃
• CAS Number: 67-66-3
• Molecular Weight: 119.38
• Beilstein/REAXYS Number: 1731042
• EC Number: 200-663-8
• MDL number: MFCD00000826
• UNSPSC Code: 12191502
• PubChem Substance ID: 329754102
• NACRES: NA.21
• assay: 99.0-99.4% (GC)
• technique(s): RNA extraction: suitable
• bp: 60.5-61.5 °C (lit.)
• vapor pressure: 160 mmHg ( 20 °C)

Properties

• agency: USP/NF; reag. ISO; reag. Ph. Eur.
• vapor density: 4.1 (vs air)
• vapor pressure: 160 mmHg ( 20 °C)
• grade: puriss. p.a.
• assay: 99.0-99.4% (GC)
• form: liquid
• contains: ~1% ethanol as stabilizer
• technique(s): RNA extraction: suitable
• impurities: ≤0.00001% free chlorine (Cl); ≤0.00005% free acid (as HCl); ≤0.0005% non-volatile matter; ≤0.005% aldehydes and ketones (as CH₃COCH₃); ≤0.005% carbonyl compounds (as CO); ≤0.01% tetrachloroethene (GC); ≤0.01% tetrachloromethane (GC); ≤0.01% trichloroethene (GC); ≤0.01% water (Karl Fischer); ≤0.03% dichloromethane (GC); 0.6-1.0% ethanol (GC)
• refractive index: n20/D 1.445 (lit.)

Description

General description

Chloroform, a halogenated hydrocarbon, is widely employed as a solvent. Its photochemical degradation has been investigated in the presence of TiO₂ aqueous suspensions (wavelength range of 310-380nm).[1]

Application

Chloroform may be employed as a solvent for the following studies:

• Extraction of RNA from plant cells.[2]
• Preparation of dioleoylphosphatidylcholine (DOPC) solution.[3]
• Dissolution of 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC).[4]

It may be employed in a rapid quantitative method for the release of periplasmic proteins from bacterial strains.[5]

Chloroform may be used as a solvent to enhance the enantioselectivity of secondary alcohols formed by the borohydride reduction of carbonyl compounds in the presence of optically active ketoiminatocobalt complexes.[6]

Other Notes

For information on chloroform miscibility, please visit the following link:
Chloroform Miscibility/Immiscibility Table

Safety Information

• pictograms: GHS06,GHS08
• signalword: Danger
• hcodes: H302,H315,H319,H331,H336,H351,H361d,H372
• pcodes: P202 - P301 + P312 - P302 + P352 - P304 + P340 + P311 - P305 + P351 + P338 - P308 + P313
• Hazard Classifications: Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Carc. 2 - Eye Irrit. 2 - Repr. 2 - Skin Irrit. 2 - STOT RE 1 Oral - STOT SE 3
• target_organs: Central nervous system, Liver,Kidney
• Storage Class: 6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
• wgk_germany: WGK 3
• flash_point_f: does not flash
• flash_point_c: does not flash