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40587

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Dichloran Glycerol Agar

suitable for microbiology, NutriSelect® Plus

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Synonym(s):
DG 18 Agar
NACRES:
NA.85

agency

according to ISO 21527-2:2008

Quality Level

sterility

non-sterile

form

powder

shelf life

limited shelf life, expiry date on the label

composition

agar, 15.0 g/L
chloramphenicol, 0.1 g/L
dextrose, 10.0 g/L
dichloran, 0.002 g/L
magnesium sulfate, 0.5 g/L
monopotassium phosphate, 1.0 g/L
peptic digest of animal tissue, 5.0 g/L

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbiological culture: suitable

final pH

5.6±0.2 (25 °C)

application(s)

bioburden testing
environmental
food and beverages

microbiology

suitability

selective for molds (General Media)
selective for yeasts (General Media)

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vibrant-m

40587

Dichloran Glycerol Agar

vibrant-m

17147

Dichloran Rose bengal Agar (Base)

vibrant-m

17222

WL Nutrient Agar

vibrant-m

91019

Schaedler Agar

sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

suitability

selective for molds (General Media), selective for yeasts (General Media)

suitability

selective for Aspergillus spp., selective for Candida spp., selective for Penicillium spp., selective for Pichia spp., selective for Saccharomyces spp., selective for Zygosaccharomyces spp., selective for molds (General Media), selective for yeasts (General Media)

suitability

nonselective and differential for Candida spp., nonselective and differential for Escherichia coli, nonselective and differential for Lactobacillus spp., nonselective and differential for Pichia spp., nonselective and differential for Proteus spp., nonselective and differential for Saccharomyces spp., nonselective and differential for Zygosaccharomyces spp., nonselective and differential for bacteria (General Media), nonselective and differential for coliforms, nonselective and differential for molds (General Media), nonselective and differential for yeasts (General Media)

suitability

anaerobic bacteria, nonselective for bacteria (General Media)

form

powder

form

powder

form

powder

form

powder

technique(s)

microbiological culture: suitable

technique(s)

-

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

agency

according to ISO 21527-2:2008

agency

according to ISO 21527-1:2008

agency

-

agency

-

General description

Dichloran Glycerol Medium Base with Chloramphenicol is recommended for selective isolation of xerophilic moulds from food samples. It is a medium for enumeration of xerophilic fungi from low-moisture foods.

Application

Dichloran Glycerol Agar has been used for culturing Aspergillus flavus AF57 on rice grains and fungal populations from conidial suspension treated rice grains.

Preparation Note

Suspend 15.8 grams in 500 ml distilled water. Heat to boiling to dissolve the medium completely. Add 110 grams of glycerol (Analytical Reagent Grade). Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Biocontrol activity of volatile-producing Bacillus megaterium and Pseudomonas protegens against Aspergillus flavus and aflatoxin production on stored rice grains
Mannaa M, et al.
Mycobiology, 45(3), 213-219 (2017)
Effect of Temperature and Relative Humidity on Growth of Aspergillus and Penicillium spp. and Biocontrol Activity of Pseudomonas protegens AS15 against Aflatoxigenic Aspergillus flavus in Stored Rice Grains
Mannaa M and Kim KD
Mycobiology, 46(3), 287-295 (2018)
A D Hocking et al.
Applied and environmental microbiology, 39(3), 488-492 (1980-03-01)
A low water activity (alpha omega) medium (0.95 alpha omega) containing 18% (wt/wt) glycerol and 2 micrograms of dichloran per ml was developed for enumerating the fungal flora of dried and semidried foods. The medium, designated DG18, was shown to

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